H2DCFDA

Name: H2DCFDA
Brand: Tocris Bioscience
SKU: 5935
Rating: 5 (2 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 5935

(2)

Citations (5)

COA / SDS

Fluorescent ROS indicator; cell permeable

Key Product Details

Description:	Fluorescent ROS indicator; cell permeable
Chemical Name:	2-[3,6-Bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic acid
Purity:	≥95% (HPLC)
Molecular Weight:	487.29

View Full Technical Details for H2DCFDA

Technical Data
Citations / Reviews (2)

Biological Activity for H2DCFDA

H2DCFDA is a fluorescent ROS indicator; becomes fluorescent when oxidized. Excitation wavelength ~490 nm, emission ~520 nm. Cell permeable.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for H2DCFDA

M. Wt	487.29
Formula	C₂₄H₁₆Cl₂O₇
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	4091-99-0
PubChem ID	77718
InChi Key	PXEZTIWVRVSYOK-UHFFFAOYSA-N
Smiles	ClC1=CC(C(C2=CC=CC=C2C(O)=O)C(C=C(Cl)C(OC(C)=O)=C3)=C3O4)=C4C=C1OC(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for H2DCFDA

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	48.73	100
	ethanol	4.87	10

Preparing Stock Solutions for H2DCFDA

The following data is based on the product molecular weight 487.29.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.05 mL	10.26 mL	20.52 mL
5 mM	0.41 mL	2.05 mL	4.10 mL
10 mM	0.21 mL	1.03 mL	2.05 mL
50 mM	0.04 mL	0.21 mL	0.41 mL

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Reconstitution Calculator

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References for H2DCFDA

References are publications that support the biological activity of the product.

Santabárbara-Ruiz ROS-induced JNK and p38 signaling is required for unpaired cytokine activation during drosophila regeneration. PLoS Genet. 2015 PMID: 26496642
Nicholson Sigma-2 receptors play a role in cellular metabolism: stimulation of glycolytic hallmarks by CM764 in human SK-N-SH neuroblastoma. J.Pharmacol.Exp.Ther. 2013 PMID: 26574517