HB-2-30

Name: HB-2-30
Brand: Tocris Bioscience
SKU: 8831

Tocris Bioscience, part of Bio-Techne | Catalog # 8831

COA / SDS

Fluorescent TG2/LRP1 substrate for imaging endocytosis

Key Product Details

Description:	Fluorescent TG2/LRP1 substrate for imaging endocytosis
Purity:	≥90% (HPLC)
Molecular Weight:	1307.59

View Full Technical Details for HB-2-30

Technical Data
Citations

Biological Activity for HB-2-30

Key information: HB-2-30 is a far-red fluorescent transglutaminase-2 (TG2) probe for imaging endocytosis in vitro and in vivo. HB-2-30 undergoes highly efficient endocytosis in complex with TG2 and α2-macroglobulin via the LRP1 pathway.

Application: Confocal microscopy.

Properties and Photophysical Data: Suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining. Excitation and emission maxima (λ) are 649 and 665 nm, respectively.

Licensing Information

Sold under license from Stanford University

Optical Data for HB-2-30

λ_abs	649 nm
λ_em	665 nm
Application	Confocal microscopy

Scientific Data Examples for HB-2-30

	*Application of HB-2-30 in vitro.* Confocal microscopy images of bone marrow-derived dendritic cells from WT C57BL/6 mice (left) and TG2KO mice (right) incubated with 3μM HB-2-30. Cells were incubated for 90 minutes with the probe before being fixed, stained for nuclei (blue), and imaged. HB-2-30 signals is in red.
	Application of HB-2-30 in vivo. Ileum tissue sections from mice dosed with 10 mg/kg HB-2-30. WT C57BL/6 mice (left) and TG2KO mice (right) were dosed with HB-2-30 and sacrificed after 2 hours. Intestines were Swiss rolled, thin sectioned, stained for nuclei (blue), and imaged via confocal fluorescence microscopy. HB-2-30 signal is in red.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for HB-2-30

M. Wt	1307.59
Formula	C₆₆H₈₆N₁₀O₁₄S₂
Single Letter Sequence	PXXPF (Modifications: Pro-1 = N-terminal Ac, X-2 = (S,E)-2-amino-7-(dimethylamino)-7-oxohept-5-enoic acid, X-3 = Dab(Sulfo-Cy5), Phe-5 = C-terminal amide)
Storage	Store at -20°C
Purity	≥90% (HPLC)
CAS Number	3031522-14-9
InChi Key	ILCHGSXJNAIGGG-AFAVFPMXSA-N
Smiles	O=C(N1[C@@H](CCC1)C(N[C@H](C(N)=O)CC2=CC=CC=C2)=O)[C@@H](NC([C@H](CC/C=C/C(N(C)C)=O)NC([C@H]3N(CCC3)C(C)=O)=O)=O)CCNC(CCCCC[N+]4=C(/C=C/C=C/C=C5C(C)(C6=CC(S(=O)(O)=O)=CC=C6N\5C)C)C(C)(C)C7=CC(S(=O)([O-])=O)=CC=C47)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for HB-2-30

Solubility

Soluble to 13.08mg/ml in DMSO

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References for HB-2-30

References are publications that support the biological activity of the product.

Loppinet Targeted lysosomal degradation of secreted and cell surface proteins through the LRP-1 pathway. J.Am.Chem.Soc. 2023 PMID: 37590164
Loppinet LRP-1 links post-translational modifications to efficient presentation of celiac disease-specific T cell antigens. Cell Chem.Biol. 2023 PMID: 36608691