HM Janelia Fluor® 526, NHS ester
Tocris Bioscience, part of Bio-Techne | Catalog # 7312
Key Product Details
| Description: | Fluorogenic spontaneously blinking yellow-emitting dye (Reactive group: NHS ester). Application: dSTORM, SMLSM, STED, SIM and confocal microscopy |
| Alternative Names: | HM-JF526 NHS |
| Chemical Name: | 2,5-Dioxopyrrolidin-1-yl 3',6'-bis(3,3-difluoroazetidin-1-yl)-2',7'-difluoro-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylate |
| Purity: | ≥90% (HPLC) |
| Molecular Weight: | 645.51 |
Biological Activity for HM Janelia Fluor® 526, NHS ester
Key information: HM Janelia Fluor® 526, NHS ester is a fluorogenic spontaneously blinking yellow-emitting dye; supplied with an NHS ester reactive group for the labeling of primary amines; hydroxymethyl derivative of JF526. Suitable for live cell imaging.
Application: Suitable for confocal microscopy, super-resolution microscopy (SRM) including dSTORM, STED, SIM and single-molecule localization spectroscopy (SMLSM). HM Janelia Fluor® 526, NHS ester is cell permeable.
Properties and Photophysical Data: The spontaneous blinking properties of HM Janelia Fluor® 526, NHS ester avoids the requirement for strongly reducing dSTORM buffers. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 526 nm and 550 nm, respectively.
To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
This product has been recently renamed. The previous name for this product was HM Janelia Fluor® 526, SE.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for HM Janelia Fluor® 526, NHS ester
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 526 nm |
| λem | 550 nm |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Application | dSTORM, SMLSM, STED, SIM, Confocal Microscopy |
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