Hoechst 33342
Tocris Bioscience, part of Bio-Techne | Catalog # 5117
Key Product Details
| Description: | Blue-fluorescent dye for DNA staining. Cell permeable. Used for: nuclear counterstain, apoptosis analysis, fixed and live-cell staining. Application: flow cytometry, confocal microscopy |
| Chemical Name: | 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride |
| Purity: | ≥98% (HPLC) |
| Molecular Weight: | 561.93 |
Biological Activity for Hoechst 33342
Key information: Hoechst 33342 is a blue-fluorescent dye for DNA staining. Cell permeable. Suitable for fixed and live-cell staining. Commonly used as a counterstain in fluorescence microscopy.
Used for: nuclear counterstain, DNA visualization, cell cycle studies, apoptosis analysis.
Application: flow cytometry, confocal microscopy.
Properties and Photophysical Data: Hoechst 33342 binds to the AT-rich regions of the minor grove in DNA which renders it specific for nuclear chromatin. Excitation and emission maxima (λ) are 350 nm and 461 nm, respectively.
Optical Data for Hoechst 33342
| Emission Color | Blue |
|---|---|
| λabs | 350 nm |
| λem | 461 nm |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Confocal Microscopy |
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Hoechst 33342 | CAS No. 875756-97-1 Fluorescent dye for staining DNA |
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