Hoechst Janelia Fluor® 646
Tocris Bioscience, part of Bio-Techne | Catalog # 6804
Key Product Details
| Description: | Fluorogenic, red-emitting DNA probe. Used for: fixed and live-cell imaging. Application: confocal microscopy, super resolution microscopy (SRM) (PALM, PAINT), lattice light sheet microscopy |
| Alternative Names: | Hoechst JF 646,JF646-Hoechst |
| Chemical Name: | 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-(2-(2-(4-(4-(5-(4-methylpiperazin-1-yl)-1H,3'H-[2,5'-bibenzo[d]imidazol]-2'-yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)benzoate |
| Purity: | ≥85% (HPLC) |
| Molecular Weight: | 1119.39 |
Biological Activity for Hoechst Janelia Fluor® 646
Key information: Hoechst Janelia Fluor® 646 is a fluorogenic red-emitting DNA probe. Suitable for fixed and live-cell imaging.
Application:confocal microscopy, super resolution microscopy (SRM) (including PALM and PAINT), lattice light sheet microscopy.
Properties and Photophysical Data: Hoechst Janelia Fluor® 646 preferentially stains and binds minor groove of AT-rich regions. The compound can be combined with fluorogenic green-emitting DNA probe Hoechst Janelia Fluor® 526 (Cat. No. 7313) for multiplexing experiments. Hoechst Janelia Fluor® 646 can also be combined with Hoechst Janelia Fluor® 526 to perform dual-color STED using the same depletion laser (λdep = 775 nm). Sub-diffraction spatial resolution STED imaging can be achieved using Hoechst Janelia Fluor® 646 in E. coli cells (~100 nM - 1 μM). Hoechst Janelia Fluor® 646 is a desirable alternative to large oligonucleotide-conjugated antibodies for PAINT experiments, particularly for bacterial studies. Excitation and emission maxima (λ) are 655 nm and 670 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1.
Hoechst Janelia Fluor® 646 is fluorogenic and fluoresces only once bound to DNA, enabling hassle-free no-wash experiments.
To measure the absorbance spectrum of Hoechst Janelia Fluo® 646 we recommend the following solvent: TFE plus 0.1% TFA.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Hoechst Janelia Fluor® 646
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 655 nm |
| λem | 670 nm |
| Extinction Coefficient (ε) | 152,000 M-1cm-1 |
| Quantum Yield (φ) | 0.54 |
| Closest Laser line | 640 nm |
| Cell Permeable | Yes |
| Application | Super Resolution Microscopy (PALM, PAINT), Confocal Microscopy, Lattice Light Sheet Microscopy |
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Application of Hoechst Janelia Fluor® 646 in U2OS cells. Fixed U2OS cells were stained with Hoechst Janelia Fluor® 646 (1 µM) for 1 hour and imaged without an intermediate washing step on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 633 nm / emission: 638–759 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus. |
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