ING-2 AM

Name: ING-2 AM
Brand: Tocris Bioscience
SKU: 7870

Tocris Bioscience, part of Bio-Techne | Catalog # 7870

COA / SDS

Fluorescent sodium ion (Na+) indicator, membrane permeable

Key Product Details

Description:	Fluorescent sodium ion (Na⁺) indicator, membrane permeable
Chemical Name:	Bis(acetoxymethyl) 3,3'-(6-(acetoxymethoxy)-4,5-dichloro-9-(3-methoxy-4-(13-(2-methoxy-4-methylphenyl)-1,4,10-trioxa-7,13-diazacyclopentadecan-7-yl)phenyl)-3-oxo-3H-xanthene-2,7-diyl)dipropionate
Purity:	≥90% (HPLC)
Molecular Weight:	1083.96

View Full Technical Details for ING-2 AM

Technical Data
Citations

Biological Activity for ING-2 AM

ING-2 AM is a membrane-permeable fluorescent sodium ion (Na⁺) indicator. It binds to sodium (K_d = 20 mM). Excitation and emission maxima (λ) are 525 and 545 nm, respectively. It displays 20-fold selectivity over K⁺ ions. It is used in in vitro and in situ to detect real-time sodium influx.

It is recommended to prepare stock solutions in DMSO.

Optical Data for ING-2 AM

λ_abs	525 nm
λ_em	545 nm
Cell Permeable	Yes
Application	Confocal microscopy, Flow cytometry, Two-photon microscopy

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Technical Data for ING-2 AM

M. Wt	1083.96
Formula	C₅₃H₆₀Cl₂N₂O₁₈
Storage	Store at -20°C
Purity	≥90% (HPLC)
CAS Number	1642554-49-1
PubChem ID	163341954
InChi Key	OTIAVQNFWAJQKZ-UHFFFAOYSA-N
Smiles	O=C(C)OCOC(CCC1=CC2=C(C3=CC(OC)=C(N4CCOCCOCCN(CCOCC4)C5=CC=C(C)C=C5OC)C=C3)C6=C(C(Cl)=C(C(CCC(OCOC(C)=O)=O)=C6)OCOC(C)=O)OC2=C(C1=O)Cl)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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References for ING-2 AM

References are publications that support the biological activity of the product.

Roder ANG-2 for quantitative Na(⁺) determination in living cells by time-resolved fluorescence microscopy. Photochem.Photobiol.Sci. 2014 PMID: 25311309
Blömer Ultrafast sodium imaging of the axon initial segment of neurons in mouse brain slices. Curr.Protoc. 2021 PMID: 33657273
Naumann Photophysical properties of Na⁺ -indicator dyes suitable for quantitative two-photon fluorescence-lifetime measurements. J.Microsc. 2018 PMID: 30191999
Gregoriades Genetic and pharmacological inactivation of apical Na⁺-K⁺-2Cl^- cotransporter 1 in choroid plexus epithelial cells reveals the physiological function of the cotransporter. Am.J.Physiol.Cell Physiol. 2019 PMID: 30576237