Janelia Fluor® 635b, NHS ester
Tocris Bioscience, part of Bio-Techne | Catalog # 8157
Key Product Details
| Description: | Blinking fluorescent dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM) including SMLM |
| Chemical Name: | 2,5-Dioxopyrrolidin-1-yl 3,7-bis(3-fluoroazetidin-1-yl)-5,5-dimethyl-3'H,5H-spiro[dibenzo[b,e]siline-10,1'-isobenzofuran]-6'-carboxylate |
| Purity: | ≥90% (HPLC) |
| Molecular Weight: | 615.71 |
Biological Activity for Janelia Fluor® 635b, NHS ester
Spontaneously Blinking Janelia Fluor® Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor® technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments.
Key Information: Janelia Fluor® 635b, NHS ester is a spontaneously blinking fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for DNA labelling using amine-terminated oligonucleotides. Also used to label oligonucleotides for super-resolution assays of transposase-accessible chromatin (ATAC-SMLM).
Application: suitable for super resolution microscopy (SRM) including single-molecule localization microscopy (SMLM).
Properties and Photophysical Data: derived from Janelia Fluor® 635, SE (Cat. No. 6419). Excitation and emission maxima (λ) are 654 nM and 671 nM, respectively.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 635b, NHS ester
| λabs | 654 nm |
|---|---|
| λem | 671 nm |
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Application of Janelia Fluor® 635b, NHS ester. SMLM RNA-FISH image of mouse embryo fibroblast cells expressing MS2 in the 3′UTR of the beta-actin gene and labeled with Janelia Fluor® 635b-oligonucleotide from Janelia Fluor® 635b, NHS ester; scale bar: 5 μm. Image kindly provided by Luke D. Lavis, Janelia Research Campus. For more information on this work read Luke D. Lavis et al (2024) A series of spontaneously blinking dyes for super-resolution microscopy. ACS Chem.Biol. bioRxiv. PMID: 38766149 |
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Application of Janelia Fluor® 635b, NHS ester. Representative 3D ATAC–SMLM rendering of accessible chromatin localizations in wild-type mouse embryonic stem (mES) cells using oligonucleotide probes labeled with Janelia Fluor® 635b, NHS ester; the color-coded localization density was calculated with a canopy radius of 250 nm; scale bars: 2 μm. Image kindly provided by Luke D. Lavis, Janelia Research Campus. For more information on this work read Luke D. Lavis et al (2024) A series of spontaneously blinking dyes for super-resolution microscopy. ACS Chem.Biol. bioRxiv. PMID: 38766149 |
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