JFX™ 554, Maleimide

Name: JFX™ 554, Maleimide
Brand: Tocris Bioscience
SKU: 8160

Tocris Bioscience, part of Bio-Techne | Catalog # 8160

COA / SDS

Deuterated Janelia Fluor® dye (Reactive group: Maleimide). Application: confocal microscopy, super-resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging

Key Product Details

Description:	Deuterated Janelia Fluor^® dye (Reactive group: Maleimide). Application: confocal microscopy, super-resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
Chemical Name:	4-((2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)-2-(3-(pyrrolidin-1-ium-1-ylidene-d₈)-6-(pyrrolidin-1-yl-d₈)-3H-xanthen-9-yl)benzoate trifluoroacetate
Purity:	≥90% (HPLC)
Molecular Weight:	734.78

View Full Technical Details for JFX™ 554, Maleimide

Technical Data
Citations

Biological Activity for JFX™ 554, Maleimide

Key Information: JFX™ 554, Maleimide is a yellow fluorescent deuterated Janelia Fluor^® dye; supplied with a Maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.

Application: Suitable for confocal microscopy, super-resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Cell permeable.

Properties and Photophysical Data: JFX™ 554, Maleimide shows enhanced brightness, photostability and chromostability. Maleimide can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag^® and SNAP-tag^®. Excitation and emission maxima (λ) are 554 nm and 576 nm, respectively; quantum yield = 0.8; extinction coefficient = 104,600 M^-1cm^-1; lactone-zwitterion equilibrium constant (K_L-Z) = 4.97.

Please see the product protocol for further information and a guide to protein/antibody labeling.

We also offer Janelia Fluor^® conjugated antibodies and custom conjugation services.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

JFX is a trademark owned by the Howard Hughes Medical Institute.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for JFX™ 554, Maleimide

λ_abs	554 nm
λ_em	576 nm
Extinction Coefficient (ε)	104,000 M^-1cm^-1
Quantum Yield (φ)	0.8
Reactive Group	Maleimide

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for JFX™ 554, Maleimide

M. Wt	734.78
Formula	C₃₅H₁₆D₁₆N₄O₆.CF₃CO₂H
Storage	Store at -20°C
Purity	≥90% (HPLC)
InChi Key	ODPVJSHHEVQADP-CXZJOTLHSA-N
Smiles	[2H]C(C([2H])([2H])C([2H])([2H])C/1([2H])[2H])([2H])[N+]1=C2C=CC3=C(C4=CC(C(NCCN5C(C=CC5=O)=O)=O)=CC=C4C([O-])=O)C6=CC=C(N7C([2H])([2H])C([2H])([2H])C([2H])([2H])C7([2H])[2H])C=C6OC3=C/2.FC(F)(C(O)=O)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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References for JFX™ 554, Maleimide

References are publications that support the biological activity of the product.

Grimm A general method to improve fluorophores using deuterated auxochromes. JACS 2021 PMID: 34056637