JFX™ 650, NHS ester

Name: JFX™ 650, NHS ester
Brand: Tocris Bioscience
SKU: 8163

Tocris Bioscience, part of Bio-Techne | Catalog # 8163

COA / SDS

Deuterated Janelia Fluor® dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging

Key Product Details

Description:	Deuterated Janelia Fluor^® dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
Chemical Name:	2-(5,5-Dimethyl-3-(pyrrolidin-1-ium-1-ylidene-d₈)-7-(pyrrolidin-1-yl-d₈)-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate
Purity:	≥90% (HPLC)
Molecular Weight:	637.86

View Full Technical Details for JFX™ 650, NHS ester

Technical Data
Citations

Biological Activity for JFX™ 650, NHS ester

Key Information: JFX^™ 650, NHS ester is a red fluorescent deuterated Janelia Fluor^®dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.

Application: Suitable for confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Cell permeable.

Properties and Photophysical Data: JFX^™ 650, NHS ester shows enhanced brightness, photostability and chromostability. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag^® and SNAP-tag^®. Excitation and emission maxima (λ) are 650 nm and 667 nm, respectively; quantum yield = 0.53; extinction coefficient = 17,600 M^-1cm^-1; lactone-zwitterion equilibrium constant (K_L-Z) = 0.015.

Please see the protocol for further information on protein/antibody labeling and conjugation.

We also offer Janelia Fluor^® conjugated antibodies and custom conjugation services.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

JFX is a trademark owned by the Howard Hughes Medical Institute.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for JFX™ 650, NHS ester

λ_abs	650 nm
λ_em	667 nm
Extinction Coefficient (ε)	17,600 M^-1cm^-1
Quantum Yield (φ)	0.53
Reactive Group	NHS ester
Reactivity	Primary amines
Cell Permeable	Yes
Application	Confocal Microscopy, dSTORM, STED, Flow Cytometry

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for JFX™ 650, NHS ester

M. Wt	637.86
Formula	C₃₅H₁₉D₁₆N₃O₆Si
Storage	Store at -20°C
Purity	≥90% (HPLC)
CAS Number	2396479-84-6
PubChem ID	153290020
InChi Key	OJCIIHUZGDFMHK-UDNRTFQASA-N
Smiles	[2H]C(C([2H])([2H])C([2H])([2H])C/1([2H])[2H])([2H])[N+]1=C2C=CC3=C(C4=CC(C(ON5C(CCC5=O)=O)=O)=CC=C4C([O-])=O)C6=CC=C(N7C([2H])([2H])C([2H])([2H])C([2H])([2H])C7([2H])[2H])C=C6[Si](C)(C)C3=C/2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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References for JFX™ 650, NHS ester

References are publications that support the biological activity of the product.

Grimm A general method to improve fluorophores using deuterated auxochromes. JACS 2021 PMID: 34056637