JQ1-FITC

Name: JQ1-FITC
Brand: Tocris Bioscience
SKU: 7722

Tocris Bioscience, part of Bio-Techne | Catalog # 7722

Citations (2)

COA / SDS

Fluorescent BET bromodomain probe; suitable for use in TR-FRET

Key Product Details

Description:	Fluorescent BET bromodomain probe; suitable for use in TR-FRET
Chemical Name:	(S)-5-(3-(1-(4-(4-Chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-2-oxo-7,10,13-trioxa-3-azahexadecan-16-yl)thioureido)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid trifluoroacetate
Purity:	≥95% (HPLC)
Molecular Weight:	1106.58

View Full Technical Details for JQ1-FITC

Technical Data
Citations

Biological Activity for JQ1-FITC

JQ1-FITC is a fluorescent BET bromodomain probe. It binds with high affinity to BRD4(BD1) and BRD4(BD2) (K_D,app values are 6.5 nM and 5.8 nM, respectively, using GST-tagged BRD4 proteins measured by CoraFluor^™ TR-FRET assay; 58.7 nM for BRD4(BD1) in a fluorescence anisotropy assay). Excitation and emission maxima (λ) are 495 nm and 525 nm.

Optical Data for JQ1-FITC

Emission Color	Green
λ_abs	495 nm
λ_em	525 nm
Extinction Coefficient (ε)	7500 M^-1cm^-1
Quantum Yield (φ)	0.92
Closest Laser line	488 nm
Application	TR-FRET, Fluorescence polarization assays
Alternative For	FITC

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for JQ1-FITC

M. Wt	1106.58
Formula	C₅₀H₅₀ClN₇O₉S₂.CF₃CO₂H
Storage	Store at -20°C
Purity	≥95% (HPLC)
InChi Key	QRVVCZSOSIMDNN-LMDYQTDMSA-N
Smiles	O=C(NCCCOCCOCCOCCCNC(NC1=CC=C(C(C(O)=O)=C1)C2=C(C(OC3=C2C=CC(O)=C3)=C4)C=CC4=O)=S)C[C@@H]5N=C(C6=CC=C(Cl)C=C6)C7=C(SC(C)=C7C)N8C5=NN=C8C.OC(C(F)(F)F)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for JQ1-FITC

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	11.07	10

Preparing Stock Solutions for JQ1-FITC

The following data is based on the product molecular weight 1106.58.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	9.04 mL	45.18 mL	90.37 mL
0.5 mM	1.81 mL	9.04 mL	18.07 mL
1 mM	0.90 mL	4.52 mL	9.04 mL
5 mM	0.18 mL	0.90 mL	1.81 mL

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References for JQ1-FITC

References are publications that support the biological activity of the product.

Payne A direct high-throughput protein quantification strategy facilitates discovery and characterization of a celastrol-derived BRD4 degrader. Cell Chem.Biol. 2022 PMID: 35649410