MitoPY1

Name: MitoPY1
Brand: Tocris Bioscience
SKU: 4428
Rating: 4 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4428

(1)

Citations (68)

COA / SDS

Fluorescent mitochondrial hydrogen peroxide indicator. Used for: live cell imaging. Application: confocal microscopy

Key Product Details

Description:	Fluorescent mitochondrial hydrogen peroxide indicator. Used for: live cell imaging. Application: confocal microscopy
Chemical Name:	[4-[4-[3-Oxo-6'-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl]-1-piperazinyl]butyl]triphenyl-phosphonium iodide
Molecular Weight:	954.68

View Full Technical Details for MitoPY1

Technical Data
Citations / Reviews (1)

Biological Activity for MitoPY1

Key information: mitochondria peroxy yellow 1 (MitoPY1) is a fluorescent probe for imaging hydrogen peroxide (H2O2) in mitochondria of living cells.

Used for: imaging hydrogen peroxide (H2O2) in mitochondria of living cells. Can also detect local increases in H2O2 in an in vitro model of Parkinson's Disease.

Application: confocal microscopy.

Properties and Photophysical Data: selective for H2O2 over superoxide, nitric oxide and hydroxyl radical.

Excitation and emission maxima (λ) are 510 nm and 530 nm, respectively, quantum yield = 0.405.

Licensing Information

Sold under license from the Regents of the University of California

Optical Data for MitoPY1

λ_abs	510 nm
λ_em	530 nm
Quantum Yield (φ)	0.405
Application	Confocal Imaging

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for MitoPY1

M. Wt	954.68
Formula	C₅₂H₅₃BIN₂O₅P
Storage	Store at -20°C
CAS Number	1041634-69-8
PubChem ID	90488942
InChi Key	JPCCULDYUUMFAD-UHFFFAOYSA-M
Smiles	O=C(O4)C5=C(C=CC=C5)C24C1=CC=C(N6CCN(CCCC[P+](C9=CC=CC=C9)(C%10=CC=CC=C%10)C8=CC=CC=C8)CC6)C=C1OC3=C2C=CC(B7OC(C)(C)C(C)(C)O7)=C3.[I-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for MitoPY1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	95.47	100

Preparing Stock Solutions for MitoPY1

The following data is based on the product molecular weight 954.68.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.05 mL	5.24 mL	10.47 mL
5 mM	0.21 mL	1.05 mL	2.09 mL
10 mM	0.10 mL	0.52 mL	1.05 mL
50 mM	0.02 mL	0.10 mL	0.21 mL

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Reconstitution Calculator

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References for MitoPY1

References are publications that support the biological activity of the product.

Zhao Lighting up H₂O₂: the molecule that is a "necessary evil" in the cell. Angew.Chem.Int.Ed.Engl. 2009 PMID: 19165849
Guo Two-photon fluorescence imaging of intracellular hydrogen peroxide with chemoselective fluorescent probes. J.Biomed.Opt. 2013 PMID: 24084856
Dickinson and Chang A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells. J.Am.Chem.Soc. 2008 PMID: 18605728