NADA-green

Name: NADA-green
Brand: Tocris Bioscience
SKU: 6648

Tocris Bioscience, part of Bio-Techne | Catalog # 6648

Citations (2)

COA / SDS

Fluorescent D-amino acid for labeling peptidoglycans in live bacteria

Key Product Details

Description:	Fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Chemical Name:	3-[(7-Nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-alanine hydrochloride
Purity:	≥95% (HPLC)
Molecular Weight:	303.66

View Full Technical Details for NADA-green

Technical Data
Citations

Biological Activity for NADA-green

NADA-green is a fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Results in strong peripheral and septal labeling of taxonomically diverse bacterial cell populations without affecting growth rate. Excitation/emission λ ~450/555 nm.

Please note that the spectral data is solvent dependant

Optical Data for NADA-green

Emission Color	Green
λ_abs	450 nm
λ_em	555 nm
Extinction Coefficient (ε)	25,000 M^-1cm^-1
Application	Fluorescence Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for NADA-green

M. Wt	303.66
Formula	C₉H₉N₅O₅.HCl
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	2253733-11-6
PubChem ID	137919868
InChi Key	NKFVSANWNYYLLB-PGMHMLKASA-N
Smiles	N[C@@H](C(O)=O)CNC1=CC=C([N+]([O-])=O)C2=NON=C12.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for NADA-green

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	30.37	100

Preparing Stock Solutions for NADA-green

The following data is based on the product molecular weight 303.66.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.29 mL	16.47 mL	32.93 mL
5 mM	0.66 mL	3.29 mL	6.59 mL
10 mM	0.33 mL	1.65 mL	3.29 mL
50 mM	0.07 mL	0.33 mL	0.66 mL

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Reconstitution Calculator

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References for NADA-green

References are publications that support the biological activity of the product.

Kuru Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ. Nat.Protoc. 2014 PMID: 25474031
Ladokhin Determining the Membrane Topology of Proteins: Insertion Pathway of a Transmembrane Helix of Annexin 12 Biochemistry 2002 PMID: 12427023
Kuru In situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids. Angew.Chem.Int.Ed. 2012 PMID: 23055266