OG 488 BAPTA-1 AM

Name: OG 488 BAPTA-1 AM
Brand: Tocris Bioscience
SKU: 6256

Tocris Bioscience, part of Bio-Techne | Catalog # 6256

COA / SDS

Cell-permeable, fluorescent Ca2+ indicator

Key Product Details

Description:	Cell-permeable, fluorescent Ca²⁺ indicator
Chemical Name:	N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[4-[[[3',6'-bis(acetyloxy)-2',7'-difluoro-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl]carbonyl]amino]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]phenoxy]ethoxy]phenyl]glycine (acetyloxy)methyl ester
Purity:	≥90% (HPLC)
Molecular Weight:	1258.06

View Full Technical Details for OG 488 BAPTA-1 AM

Technical Data
Citations

Biological Activity for OG 488 BAPTA-1 AM

OG 488 BAPTA-1 AM is a cell-permeable, fluorescently labeled Ca²⁺ indicator (K_d =170 nM). Composed of a green fluorescent probe (OG 488, Cat. No. 6257) conjugated to a cell-permeable Ca²⁺ chelator (BAPTA AM, Cat. No. 2787). Fluorescent intensity increases approximately 14-fold from resting intensity, upon Ca²⁺ binding. Displays uniform cellular distribution with minimal subcellular compartmentalization. Excitation/emission λ 494/523 nm.

Optical Data for OG 488 BAPTA-1 AM

Emission Color	Green
λ_abs	494 nm
λ_em	523 nm
Extinction Coefficient (ε)	19000 M^-1cm^-1
Quantum Yield (φ)	0.91
Closest Laser line	488 nm
Cell Permeable	Yes
Application	Flow Cytometry, Fluorescence Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for OG 488 BAPTA-1 AM

M. Wt	1258.06
Formula	C₅₉H₅₃F₂N₃O₂6
Storage	Store at -20°C
Purity	≥90% (HPLC)
CAS Number	244167-57-5
PubChem ID	102028682
InChi Key	NQVWLDWXFNDFLA-UHFFFAOYSA-N
Smiles	FC1=CC2=C(C3=CC=C(C(NC4=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC5=CC=CC=C5N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C4)=O)C=C3C([O-])=O)C6=CC(F)=C(OC(C)=O)C=C6[O+]=C2C=C1OC(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for OG 488 BAPTA-1 AM

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	1.26	1

Preparing Stock Solutions for OG 488 BAPTA-1 AM

The following data is based on the product molecular weight 1258.06.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.01 mM	79.49 mL	397.44 mL	794.87 mL
0.05 mM	15.90 mL	79.49 mL	158.97 mL
0.1 mM	7.95 mL	39.74 mL	79.49 mL
0.5 mM	1.59 mL	7.95 mL	15.90 mL

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References for OG 488 BAPTA-1 AM

References are publications that support the biological activity of the product.

Thomas A comparison of fluorescent Ca²⁺ indicator properties and their use in measuring elementary and global Ca²⁺ signals Cell Calcium 2000 PMID: 11032777