Peroxy Orange 1

Name: Peroxy Orange 1
Brand: Tocris Bioscience
SKU: 4944

Tocris Bioscience, part of Bio-Techne | Catalog # 4944

Citations (15)

COA / SDS

Fluorescent hydrogen peroxide indicator

Key Product Details

Description:	Fluorescent hydrogen peroxide indicator
Alternative Names:	PO1
Chemical Name:	2',3',6',7'-Tetrahydro-12'-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-spiro[isobenzofuran-1(3H),9'-[1H,5H,9H]xantheno[2,3,4-ij]quinolizin]-3-one
Molecular Weight:	521.41

View Full Technical Details for Peroxy Orange 1

Technical Data
Citations

Biological Activity for Peroxy Orange 1

Peroxy Orange 1 is a fluorescent probe for imaging hydrogen peroxide (H₂O₂); cell permeable. Displays an orange intracellular fluorescence in response to H₂O₂ signals produced in RAW 264.7 macrophages and A431 cells during immune response and growth factor stimulation, respectively. Excitation wavelength 543 nm, emission between 545 and 750 nm.

Licensing Information

Sold under license from the Regents of the University of California

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Technical Data for Peroxy Orange 1

M. Wt	521.41
Formula	C₃₂H₃₂BNO₅
Storage	Store at -20°C
CAS Number	1199576-10-7
PubChem ID	44546671
InChi Key	KNTNXZHHCPNNGB-UHFFFAOYSA-N
Smiles	O=C(O1)C2=C(C=CC=C2)C31C4=CC5=C6C(CCCN6CCC5)=C4OC7=C3C=CC(B8OC(C)(C)C(C)(C)O8)=C7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Peroxy Orange 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	5.21	10

Preparing Stock Solutions for Peroxy Orange 1

The following data is based on the product molecular weight 521.41.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	19.18 mL	95.89 mL	191.79 mL
0.5 mM	3.84 mL	19.18 mL	38.36 mL
1 mM	1.92 mL	9.59 mL	19.18 mL
5 mM	0.38 mL	1.92 mL	3.84 mL

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References for Peroxy Orange 1

References are publications that support the biological activity of the product.

Dickinson Chemistry and biology of reactive oxygen species in signaling or stress responses. Nat.Chem.Biol. 2011 PMID: 21769097
Woolley H₂O₂ production downstream of FLT3 is mediated by p22phox in the endoplasmic reticulum and is required for STAT5 signalling. PLoS ONE 2012 PMID: 22807997
Dickinson A palette of fluorescent probes with varying emission colors for imaging hydrogen peroxide signaling in living cells. J.Am.Chem.Soc. 2010 PMID: 20361787