Phalloidin-TRITC

Name: Phalloidin-TRITC
Brand: Tocris Bioscience
SKU: 5783

Tocris Bioscience, part of Bio-Techne | Catalog # 5783

COA / SDS

Red-orange fluorescent cytoskeleton stain. Binds and labels F-actin. Used for: F-actin staining. Application: fluorescent microscopy, immunofluorescence (IHC, ICC)

Key Product Details

Description:	Red-orange fluorescent cytoskeleton stain. Binds and labels F-actin. Used for: F-actin staining. Application: fluorescent microscopy, immunofluorescence (IHC, ICC)
Chemical Name:	7-[(4R)-5-[[[[4-[3,6-Bis(dimethylamino)xanthylium-9-yl]-3-carboxyphenyl]amino]thioxomethyl]amino]-4-hydroxy-L-leucine]phalloidin
Purity:	≥90% (HPLC)
Molecular Weight:	1231.4

View Full Technical Details for Phalloidin-TRITC

Technical Data
Citations

Biological Activity for Phalloidin-TRITC

Key information: Phalloidin-TRITC is a red-orange fluorescent cytoskeleton stain. It binds and labels specifically F-actin.

Used for: Phalloidin-TRITC can be used to visualize and quantify F-actin in tissue sections, cell cultures or cell-free experiments with optimal results in formaldehyde-fixed and permeabilized cells.

Application: fluorescent microscopy, immunofluorescence (IHC, ICC).

Properties and Photophysical Data: Phalloidin-TRITC is composed of the F-actin probe, Phalloidin (Cat. No. 4535), conjugated to Tetramethylrhodamine isothiocyanate (TRITC) dye. Phalloidin binds F-actin with high selectivity allowing Phalloidin-TRITC to stain actin with high-contrast discrimination. Excitation and emission maxima (λ) are 540 nm and 565 nm, respectively.

We suggest making up a stock solution of this product by dissolving the vial contents in 1.5 mL methanol or DMSO. Please refer to the protocol for further information on using this product.

Scientific Data Examples for Phalloidin-TRITC

	Applications in HeLa Cells for Phalloidin-TRITC. Application of Phalloidin-TRITC: HeLa cells were labeled with 150 nM Phalloidin-TRITC for 20 minutes. Counterstained with DAPI (Catalog # 5748).

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for Phalloidin-TRITC

M. Wt	1231.4
Formula	C₆₀H₇₀N₁₂O₁₃S₂
Storage	Store at -20°C
Purity	≥90% (HPLC)
CAS Number	915013-10-4
Smiles	C[C@H](O)[C@H]1NC(=O)C(C)NC(=O)[C@H](C[C@@](C)(O)CNC(=S)NC2=CC(C([O-])=O)=C(C=C2)C2=C3C=CC(C=C3OC3=C2C=CC(=C3)N(C)C)=[N+](C)C)NC(=O)[C@@H]2CC3=C(NC4=C3C=CC=C4)SC[C@@H](NC1=O)C(=O)N1C[C@H](O)C[C@H]1C(=O)N[C@@H](C)C(=O)N2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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Product Documents for Phalloidin-TRITC

COA

Certificate of Analysis

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