Rf470DL

Name: Rf470DL
Brand: Tocris Bioscience
SKU: 7406

Tocris Bioscience, part of Bio-Techne | Catalog # 7406

Citations (1)

COA / SDS

Blue rotor-fluorogenic fluorescent D-amino acid for labeling peptidoglycans in live bacteria

Key Product Details

Description:	Blue rotor-fluorogenic fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Chemical Name:	N⁶-[3-[6-[5-[2-Carboxy-2-cyanoethenyl]-2-thienyl]-3,4-dihydro-1(2H)-quinolinyl]-1-oxopropyl]-D-lysine hydrochloride
Purity:	≥95% (HPLC)
Molecular Weight:	547.07

View Full Technical Details for Rf470DL

Technical Data
Citations

Biological Activity for Rf470DL

Rf470DL is a blue rotor-fluorogenic fluorescent D-amino acid for labeling peptidoglycans in live bacteria. Rf470DL fluoresces only when it becomes incorporated into the peptidoglycan, enabling no wash experiments. Excitation/emission λ ~470/620 nm; quantum yield = 0.042; extinction coefficient 33,106 M-¹ cm-¹ (measured in PBS containing 50% glycerol).

Licensing Information

Sold under license from Indiana University. U.S. patent application number 16/967,874.

Optical Data for Rf470DL

Emission Color	Red
λ_abs	470 nm
λ_em	640 nm
Extinction Coefficient (ε)	33106 M^-1cm^-1
Quantum Yield (φ)	0.042
Application	Fluorogenic Probe for Imaging Bacterial Cell Wall Biosynthesis (PG Biosynthesis)

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for Rf470DL

M. Wt	547.07
Formula	C₂₆H₃₀N₄O₅S.HCl
Storage	Store at -20°C
Purity	≥95% (HPLC)
InChi Key	ITTDOARQMPIHGH-IVUCCOTMSA-N
Smiles	N[C@@H](C(O)=O)CCCCNC(CCN1CCCC2=CC(C3=CC=C(/C=C(C(O)=O)\C#N)S3)=CC=C12)=O.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Rf470DL

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	54.71	100

Preparing Stock Solutions for Rf470DL

The following data is based on the product molecular weight 547.07.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.83 mL	9.14 mL	18.28 mL
5 mM	0.37 mL	1.83 mL	3.66 mL
10 mM	0.18 mL	0.91 mL	1.83 mL
50 mM	0.04 mL	0.18 mL	0.37 mL

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References for Rf470DL

References are publications that support the biological activity of the product.

Hsu Fluorogenic D-amino acids enable real-time monitoring of peptidoglycan biosynthesis and high-throughput transpeptidation assays. Nat.Chem. 2019 PMID: 30804500