SAHA-FITC

Name: SAHA-FITC
Brand: Tocris Bioscience
SKU: 7970

Tocris Bioscience, part of Bio-Techne | Catalog # 7970

COA / SDS

Fluorescent HDAC1/2 probe; suitable for use in TR-FRET and FP assays

Key Product Details

Description:	Fluorescent HDAC1/2 probe; suitable for use in TR-FRET and FP assays
Chemical Name:	2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-5-(3-(4-(8-(hydroxyamino)-8-oxooctanamido)benzyl)thioureido)benzoic acid
Purity:	≥90% (HPLC)
Molecular Weight:	682.75

View Full Technical Details for SAHA-FITC

Technical Data
Citations

Biological Activity for SAHA-FITC

SAHA-FITC is a fluorescent HDAC1/2 probe for use as a TR-FRET acceptor for CoraFluor labeling and in fluorescence polarization binding assays. Binds non-selectively to HDAC1 and HDAC2 (K_d values are 56 nM and 132 nM respectively; K_i = 1.5 nM).

Optical Data for SAHA-FITC

Emission Color	Green
λ_abs	495 nm
λ_em	525 nm

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for SAHA-FITC

M. Wt	682.75
Formula	C₃₆H₃₄N₄O₈S
Storage	Store at -20°C
Purity	≥90% (HPLC)
CAS Number	1160823-10-8
PubChem ID	44123666
InChi Key	GQKWRDWQZXNRAB-UHFFFAOYSA-N
Smiles	OC(C=C1OC2=C3)=CC=C1C(C4=CC=C(NC(NCC5=CC=C(NC(CCCCCCC(NO)=O)=O)C=C5)=S)C=C4C(O)=O)=C2C=CC3=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SAHA-FITC

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	6.83	10

Preparing Stock Solutions for SAHA-FITC

The following data is based on the product molecular weight 682.75.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	14.65 mL	73.23 mL	146.47 mL
0.5 mM	2.93 mL	14.65 mL	29.29 mL
1 mM	1.46 mL	7.32 mL	14.65 mL
5 mM	0.29 mL	1.46 mL	2.93 mL

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Reconstitution Calculator

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References for SAHA-FITC

References are publications that support the biological activity of the product.

Payne and Mazitschek Resolving the deceptive isoform and complex selectivity of HDAC1/2 inhibitors. Cell Chem.Biol. 2022 PMID: 35298895
Kral Divergent kinetics differentiate the mechanism of action of two HDAC inhibitors. Biochemistry 2014 PMID: 24450491