Sulfidefluor 7 AM

Name: Sulfidefluor 7 AM
Brand: Tocris Bioscience
SKU: 4943

Tocris Bioscience, part of Bio-Techne | Catalog # 4943

Citations (10)

COA / SDS

H2S fluorescent probe

Key Product Details

Description:	H₂S fluorescent probe
Chemical Name:	N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[(3',6'-diazido-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]glycine (acetyloxy)methyl ester
Molecular Weight:	685.55

View Full Technical Details for Sulfidefluor 7 AM

Technical Data
Citations

Biological Activity for Sulfidefluor 7 AM

Sulfidefluor 7 AM is a reported to be a hydrogen sulphide (H₂S) fluorescent probe. Contains an acetoxymethyl ester, which enhances cell permeability and is hydrolyzed inside the cell to trap the probe. Used to visualize VEGF-induced H₂S production in human umbilical vein endothelial cells (HUVECS). Excitation wavelength ~ 495 nm; emission maximum ~ 520 nm.

Licensing Information

Sold under license from the Regents of the University of California

Optical Data for Sulfidefluor 7 AM

Emission Color	Green
λ_abs	495 nm
λ_em	520 nm
Cell Permeable	Yes
Application	Fluorescence Microscopy

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for Sulfidefluor 7 AM

M. Wt	685.55
Formula	C₃₁H₂₃N₇O₁₂
Storage	Store at -20°C
CAS Number	1416872-50-8
PubChem ID	89306941
InChi Key	QIAZHSBWGCYJGQ-UHFFFAOYSA-N
Smiles	O=C(O1)C2=C(C=CC(C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=O)=C2)C31C4=CC=C(N=[N+]=[N-])C=C4OC5=C3C=CC(N=[N+]=[N-])=C5

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Sulfidefluor 7 AM

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	13.71	20

Preparing Stock Solutions for Sulfidefluor 7 AM

The following data is based on the product molecular weight 685.55.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	7.29 mL	36.47 mL	72.93 mL
1 mM	1.46 mL	7.29 mL	14.59 mL
2 mM	0.73 mL	3.65 mL	7.29 mL
10 mM	0.15 mL	0.73 mL	1.46 mL

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References for Sulfidefluor 7 AM

References are publications that support the biological activity of the product.

Lippert Reaction-based fluorescent probes for selective imaging of hydrogen sulfide in living cells. J.Am.Chem.Soc. 2011 PMID: 21671682
Lin Cell-trappable fluorescent probes for endogenous hydrogen sulfide signaling and imaging H₂O₂-dependent H₂S production. Proc.Natl.Acad.Sci.USA. 2013 PMID: 23589874