Thalidomide-Cyanine 5

Name: Thalidomide-Cyanine 5
Brand: Tocris Bioscience
SKU: 7288

Tocris Bioscience, part of Bio-Techne | Catalog # 7288

Citations (85)

COA / SDS

High affinity cereblon fluorescent probe

Key Product Details

Description:	High affinity cereblon fluorescent probe
Chemical Name:	2-((1E,3E)-5-((E)-1-(6-((4-(2-((2-(2,6-Dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)butyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)penta-1,3-dien-1-yl)-1-ethyl-3,3-dimethyl-3H-indol-1-ium-5-sulfonate
Purity:	≥90% (HPLC)
Molecular Weight:	1041.2

View Full Technical Details for Thalidomide-Cyanine 5

Technical Data
Citations

Biological Activity for Thalidomide-Cyanine 5

Thalidomide-Cyanine 5 is a fluorescent probe for cereblon E3 ligases. Binds with high affinity to DDB1-CRBN. Suitable for use in TR-FRET.

Optical Data for Thalidomide-Cyanine 5

Emission Color	Red
λ_abs	650 nm
λ_em	665 nm
Closest Laser line	647 nm
Application	TR-FRET

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for Thalidomide-Cyanine 5

M. Wt	1041.2
Formula	C₅₂H₆₀N₆O₁₃S₂
Storage	Store at -20°C
Purity	≥90% (HPLC)
InChi Key	RWTYSFWRLGGPLR-UHFFFAOYSA-N
Smiles	O=C1N(C2CCC(NC2=O)=O)C(C3=CC=CC(OCC(NCCCCNC(CCCCCN(C(C=CC(S(=O)(O)=O)=C4)=C4C/5(C)C)C5=C\C=C\C=C\C6=[N+](CC)C7=C(C=C(S(=O)([O-])=O)C=C7)C6(C)C)=O)=O)=C31)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Thalidomide-Cyanine 5

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	20.82	20

Preparing Stock Solutions for Thalidomide-Cyanine 5

The following data is based on the product molecular weight 1041.2.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	4.80 mL	24.01 mL	48.02 mL
1 mM	0.96 mL	4.80 mL	9.60 mL
2 mM	0.48 mL	2.40 mL	4.80 mL
10 mM	0.10 mL	0.48 mL	0.96 mL

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References for Thalidomide-Cyanine 5

References are publications that support the biological activity of the product.

Chessum Demonstrating in-cell target engagement using a pirin protein degradation probe (CCT367766). J.Med.Chem. 2018 PMID: 29240418
Petzold Structural basis of lenalidomide-induced CK1α degradation by the CRL4^CRBN ubiquitin ligase. Nature 2016 PMID: 26909574
Cavadini Cullin-RING ubiquitin E3 ligase regulation by the COP9 signalosome. Nature 2016 PMID: 27029275
Fischer Structure of the DDB1-CRBN E3 ubiquitin ligase in complex with thalidomide. Nature 2014 PMID: 25043012