Trolox

Name: Trolox
Brand: Tocris Bioscience
SKU: 6002

Tocris Bioscience, part of Bio-Techne | Catalog # 6002

Citations (18)

COA / SDS

Antifade reagent; antioxidant vitamin E derivative; cell permeable and water soluble

Key Product Details

Description:	Antifade reagent; antioxidant vitamin E derivative; cell permeable and water soluble
Chemical Name:	3,4-Dihydro-6-hydroxy-2,5,7,8-tetramethyl-2H-1-benzopyran-2-carboxylic acid
Purity:	≥98% (HPLC)
Molecular Weight:	250.29

View Full Technical Details for Trolox

Technical Data
Citations

Biological Activity for Trolox

Trolox is an antioxidant vitamin E derivative; suppresses osteoclast formation and inhibits IL-1-induced osteoclast bone loss. Also inhibits ferroptosis. Commonly used antifade reagent in live cell imaging; suppresses blinking and photobleaching. Cell-permeable and water soluble.

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Technical Data for Trolox

M. Wt	250.29
Formula	C₁₄H₁₈O₄
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	53188-07-1
PubChem ID	40634
InChi Key	GLEVLJDDWXEYCO-UHFFFAOYSA-N
Smiles	CC1=C(C(C)=C2CCC(OC2=C1C)(C(O)=O)C)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Trolox

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	25.03	100
	ethanol	25.03	100

Preparing Stock Solutions for Trolox

The following data is based on the product molecular weight 250.29.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.00 mL	19.98 mL	39.95 mL
5 mM	0.80 mL	4.00 mL	7.99 mL
10 mM	0.40 mL	2.00 mL	4.00 mL
50 mM	0.08 mL	0.40 mL	0.80 mL

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References for Trolox

References are publications that support the biological activity of the product.

Cordes On the mechanism of Trolox as antiblinking and antibleaching reagent. J.Am.Chem.Soc. 2009 PMID: 19301868
Lee Trolox prevents osteoclastogenesis by suppressing RANKL expression and signaling. J.Biol.Chem. 2009 PMID: 19299513
Xie Ferroptosis: process and function. Cell Death Differ. 2016 PMID: 26794443
Giulivi Inhibition of protein radical reactions of ferrylmyoglobin by the water-soluble analog of vitamin E, Trolox C. Arch.Biochem.Biophys. 1993 PMID: 8489259