YADA

Name: YADA
Brand: Tocris Bioscience
SKU: 6650

Tocris Bioscience, part of Bio-Techne | Catalog # 6650

COA / SDS

Green-yellow lucifer yellow-based fluorescent D-amino acid for labeling peptidoglycans in live bacteria

Key Product Details

Description:	Green-yellow lucifer yellow-based fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Chemical Name:	(αR)-α,6-Diamino-1,3-dioxo-5,8-disulfo-1H-benz[de]isoquinoline-2(3H)-propanoic acid
Purity:	≥95% (HPLC)
Molecular Weight:	459.41

View Full Technical Details for YADA

Technical Data
Citations

Biological Activity for YADA

YADA is a green-yellow lucifer yellow-based fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Exhibits large stokes shift and wider emission spectra than other FDAAs, allowing excitation via a violet light source and detection with a green filter. Displays good water solubility, photostability and thermostability. Excitation/emission λ ~426/535 nm.

Optical Data for YADA

Emission Color	Green-yellow
λ_abs	426 nm
λ_em	535 nm
Extinction Coefficient (ε)	24200 M^-1cm^-1
Quantum Yield (φ)	0.2
Application	Confocal Microscopy, Super Resolution Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for YADA

M. Wt	459.41
Formula	C₁₅H₁₃N₃O₁₀S₂
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	1471982-33-8
PubChem ID	134159307
InChi Key	YXWYHJBYHLJEIR-UHFFFAOYSA-N
Smiles	O=C(N(C[C@@H](N)C(O)=O)C1=O)C2=C(C1=CC(S(=O)(O)=O)=C3N)C3=CC(S(=O)(O)=O)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for YADA

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	45.94	100

Preparing Stock Solutions for YADA

The following data is based on the product molecular weight 459.41.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.18 mL	10.88 mL	21.77 mL
5 mM	0.44 mL	2.18 mL	4.35 mL
10 mM	0.22 mL	1.09 mL	2.18 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for YADA

References are publications that support the biological activity of the product.

Hsu Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem.Sci. 2017 PMID: 28989665