Rabbit IgG Isotype Control
Novus Biologicals, part of Bio-Techne | Catalog # NB810-56910
Key Product Details
Species Reactivity
Rabbit
Applications
Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
5 mg/ml
Product Specifications
Specificity
By immunoelectrophoresis the IgG was shown to 1) react with antiserum specific for rabbit IgG, 2) not react with antiserum specific for IgA or IgM, and 3) produce a single precipitin arc with antiserum against rabbit serum identical to that produced with anti-IgG antisera.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody.
Scientific Data Images for Rabbit IgG Isotype Control
![Immunohistochemistry-Frozen: Rabbit IgG Isotype Control [NB810-56910]](https://resources.bio-techne.com/images/products/Rabbit-IgG-Isotype-Control-Immunohistochemistry-Frozen-NB810-56910-img0012.jpg "Immunohistochemistry-Frozen: Rabbit IgG Isotype Control [NB810-56910]")
Immunohistochemistry-Frozen: Rabbit IgG Isotype Control [NB810-56910]
Immunohistochemistry-Frozen: Rabbit IgG Isotype Control [NB810-56910] - Mouse ear skin (aceton-fixed frozen section) stained with rabbit IgG isotype control, which was used as a negative control. Image from verified customer review.![Immunohistochemistry-Frozen: Rabbit IgG Isotype Control [NB810-56910]](https://resources.bio-techne.com/images/products/Rabbit-IgG-Isotype-Control-Immunohistochemistry-Frozen-NB810-56910-img0011.jpg "Immunohistochemistry-Frozen: Rabbit IgG Isotype Control [NB810-56910]")
Immunohistochemistry-Frozen: Rabbit IgG Isotype Control [NB810-56910]
Immunohistochemistry-Frozen: Rabbit IgG Isotype Control [NB810-56910] - Mouse ear skin (aceton-fixed frozen section) stained with rabbit IgG isotype control, which was used as a negative control. Image from verified customer review.Applications for Rabbit IgG Isotype Control
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:10-1:500
Flow Cytometry
1:10 - 1:1000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunoprecipitation
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
IP usage was reported in scientific literature (PMID: 23872598). ChIP usage was reported in scientific literature (PMID: 24324133). Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined. Use in IHC reported in secitific publication PMID: 32736983
Reviewed Applications
Read 6 reviews rated 5 using NB810-56910 in the following applications:
Formulation, Preparation, and Storage
Purification
Ion exchange chromatography
Formulation
PBS.
Preservative
0.09% Sodium Azide
Concentration
5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: IgG
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Gene Symbol
IGHG1
Additional IgG Products
Product Documents for Rabbit IgG Isotype Control
Product Specific Notices for Rabbit IgG Isotype Control
Protein concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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