GTF2H2 293T Cell Transient Overexpression Lysate, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # H00002966-T02

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H00002966-T02
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Key Product Details

Species

Human

Applications

Western Blot

Product Summary for GTF2H2 293T Cell Transient Overexpression Lysate

Quality control test: Transient overexpression cell lysate was tested with Anti-GTF2H2antibody by Western Blot. Plasmid: pCMV-GTF2H2 full-length

Product Specifications

Specificity

GTF2H2 293T Cell Transient Overexpression Lysate(Denatured)

TMW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

293T Cell Transient Overexpression

Protein State

Denatured

Formulation, Preparation, and Storage

Formulation

1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Concentration

mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: GTF2H2

Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFII. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of five subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and MAT1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.

Alternate Names

Basic transcription factor 2 44 kDa subunit, BTF2, BTF2 p44, BTF2P44TFIIH basal transcription factor complex p44 subunit, General transcription factor IIH polypeptide 2, general transcription factor IIH subunit 2, general transcription factor IIH, polypeptide 2 (44kD subunit), general transcription factor IIH, polypeptide 2, 44kD subunit, general transcription factor IIH, polypeptide 2, 44kDa, MGC102806, T-BTF2P44, TFIIH

Gene Symbol

GTF2H2

Additional GTF2H2 Products

Product Documents for GTF2H2 293T Cell Transient Overexpression Lysate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GTF2H2 293T Cell Transient Overexpression Lysate

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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