Recombinant Mouse CCL8/MCP-2 Protein

Name: Recombinant Mouse CCL8/MCP-2 Protein
Brand: Novus Biologicals
SKU: NBP2-35159
Rating: 5 (1 reviews)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35159

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Key Product Details

Source

E. coli

Accession #

Q9Z121

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL8/MCP-2 Proteins and Enzymes »

Product Specifications

Description

A single, non-glycosylated polypeptide chain containing 74 amino acids corresponding to CCL8/MCP-2 Source: E. coli

Uniprot ID: Q9Z121

Amino Acid Sequence: GPDKAPVTCC FHVLKLKIPL RVLKSYERIN NIQCPMEAVV FQTKQGMSLC VDPTQKWVSE YMEILDQKSQ ILQP

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL8/MCP-2 as determined by LAL method.

Predicted Molecular Mass

8.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCL8/MCP-2 protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml

Protein / Peptide Type

Recombinant Protein

Reviewed Applications

Read 1 review rated 5 using NBP2-35159 in the following applications:

Immunoassay Standard (1 Review)

Formulation, Preparation and Storage

NBP2-35159

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL8/MCP-2

Murine CCL8, also known as monocyte chemotactic protein 2 (MCP-2), is belonging to the CC chemokine family and is encoded by the gene CCL8 in mouse. MCP-2 has two homogeneous MCP-1 (CCL2) and MCP-3 (CCL7). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL8 shares 62 % amino acid sequence identity with MCP-1, and shares 58 % amino acid sequence identity with MCP-2. CCL8 has chemotactic fuction for monocytes, eosinophils and neutrophils. In addition, it can also chemoattract activated NK cells.

Alternate Names

MCP-2, MCP2, CCL8, MCP-2/CCL8

Gene Symbol

CCL8

UniProt