Recombinant Human Chondromodulin-1/LECT1 His Protein

Name: Recombinant Human Chondromodulin-1/LECT1 His Protein
Brand: Novus Biologicals
SKU: NBP2-51539

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-51539

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Chondromodulin-1 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 214-334 of Human Chondromodulin-1/LECT1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSREVVRKI VPTTTKRPHS GPRSNPGAGR LNNETRPSVQ EDSQAFNPDN PYHQQEGESM TFDPRLDHEG ICCIECRRSY THCQKICEPL GGYYPWPYNY QGCRSACRVI MPCSWWVARI LGMV

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

16.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Chondromodulin-1/LECT1 His Protein

SDS-PAGE: Recombinant Human Chondromodulin-1/LECT1 His Protein [NBP2-51539]

SDS-Page: Recombinant Human Chondromodulin-1/LECT1 Protein [NBP2-51539] - 15% SDS Page (3 ug)

Formulation, Preparation and Storage

NBP2-51539

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Chondromodulin-1

LECT1 encodes a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein although no biological activity has yet been defined for it. The C-terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell-derived chemotaxin-1 or chondromodulin-1. The mature protein promotes chondrocyte growth and inhibits angiogenesis. This gene is expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone. It may be involved also in the broad control of tissue vascularization during development. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

BRICD3, ChM-1, Chondromodulin1, LECT1

Gene Symbol

LECT1

Additional Chondromodulin-1 Products

Product Documents for Recombinant Human Chondromodulin-1/LECT1 His Protein

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COA

Product Specific Notices for Recombinant Human Chondromodulin-1/LECT1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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