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Recombinant Human GAD1/GAD67 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-51849

Novus Biologicals, part of Bio-Techne
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NBP2-51849-0.1mg
NBP2-51849-0.5mg

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-594 of Human GAD1/GAD67

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMASSTPS SSATSSNAGA DPNTTNLRPT TYDTWCGVAH GCTRKLGLKI CGFLQRTNSL EEKSRLVSAF KERQSSKNLL SCENSDRDAR FRRTETDFSN LFARDLLPAK NGEEQTVQFL LEVVDILLNY VRKTFDRSTK VLDFHHPHQL LEGMEGFNLE LSDHPESLEQ ILVDCRDTLK YGVRTGHPRF FNQLSTGLDI IGLAGEWLTS TANTNMFTYE IAPVFVLMEQ ITLKKMREIV GWSSKDGDGI FSPGGAISNM YSIMAARYKY FPEVKTKGMA AVPKLVLFTS EQSHYSIKKA GAALGFGTDN VILIKCNERG KIIPADFEAK ILEAKQKGYV PFYVNATAGT TVYGAFDPIQ EIADICEKYN LWLHVDAAWG GGLLMSRKHR HKLNGIERAN SVTWNPHKMM GVLLQCSAIL VKEKGILQGC NQMCAGYLFQ PDKQYDVSYD TGDKAIQCGR HVDIFKFWLM WKAKGTVGFE NQINKCLELA EYLYAKIKNR EEFEMVFNGE PEHTNVCFWY IPQSLRGVPD SPQRREKLHK VAPKIKALMM ESGTTMVGYQ PQGDKANFFR MVISNPAATQ SDIDFLIEEI ERLGQDL

Purity

>80%, by SDS-PAGE

Predicted Molecular Mass

69.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GAD1/GAD67 His Protein

SDS-PAGE: Recombinant Human GAD1/GAD67 His Protein [NBP2-51849]

SDS-PAGE: Recombinant Human GAD1/GAD67 His Protein [NBP2-51849]

SDS-Page: Recombinant Human GAD1/GAD67 Protein [NBP2-51849] - 15% SDS Page (3 ug)

Formulation, Preparation and Storage

NBP2-51849
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAD1/GAD67

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) that are encoded by two different genes and appear to have distinct intraneuronal distribution in the brain. This antibody reacts with GAD67 and is predicted to react with GAD65.

Long Name

Glutamate Decarboxylase 1 (Brain, 67kDa)

Alternate Names

GAD67

Gene Symbol

GAD1

Additional GAD1/GAD67 Products

Product Documents for Recombinant Human GAD1/GAD67 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human GAD1/GAD67 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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