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Recombinant Human MAGP-1/MFAP2 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23234

Novus Biologicals, part of Bio-Techne
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NBP2-23234

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 18-183 of Human MAGP-1/MFAP2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMQGQYDL DPLPPFPDHV QYTHYSDQID NPDYYDYQEV TPRPSEEQFQ FQSQQQVQQE VIPAPTPEPG NAELEPTEPG PLDCREEQYP CTRLYSIHRP CKQCLNEVCF YSLRRVYVIN KEICVRTVCA HEELLRADLC RDKFSKCGVM ASSGLCQSVA ASCARSCGSC

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

21.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MAGP-1/MFAP2 His Protein

SDS-PAGE: Recombinant Human MAGP-1/MFAP2 His Protein [NBP2-23234]

SDS-PAGE: Recombinant Human MAGP-1/MFAP2 His Protein [NBP2-23234]

SDS-Page: Recombinant Human MAGP-1/MFAP2 Protein [NBP2-23234]

Formulation, Preparation and Storage

NBP2-23234
Formulation 20 mM Tris-HCl buffer (pH 8.0), 1 M UREA, 20% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAGP-1/MFAP2

Microfibrillar-associated protein 2, also known as MFAP2, is an O-glycosylated protein secreted to the extracellular space and the extracellular matrix. MFAP2 associates with biglycan and elastin in a ternary complex. It is shown to play a significant role in the support and distensibility of the juxtacanalicular region of these collector channels. It is also reported to inhibit LTB-1 binding to fibrillin-1, stimulate the phosphorylation of Smad2, and thereby mediate the subsequent extracellular deposition of latent TGFbeta. Recombinant human MFAP2, fused to His-tag at N-terminus, was expressed in E.coli.

Long Name

Microfibril-associated Glycoprotein 1

Alternate Names

MAGP1, MFAP2

Gene Symbol

MFAP2

Additional MAGP-1/MFAP2 Products

Product Documents for Recombinant Human MAGP-1/MFAP2 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human MAGP-1/MFAP2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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