Recombinant Human NT5C2 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72471

Novus Biologicals, part of Bio-Techne
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NBP1-72471-0.5mg
NBP1-72471-100ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all NT5C2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-561 of Human NT5C2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSTSWSDRLQ NAADMPANMD KHALKKYRRE AYHRVFVNRS LAMEKIKCFG FDMDYTLAVY KSPEYESLGF ELTVERLVSI GYPQELLSFA YDSTFPTRGL VFDTLYGNLL KVDAYGNLLV CAHGFNFIRG PETREQYPNK FIQRDDTERF YILNTLFNLP ETYLLACLVD FFTNCPRYTS CETGFKDGDL FMSYRSMFQD VRDAVDWVHY KGSLKEKTVE NLEKYVVKDG KLPLLLSRMK EVGKVFLATN SDYKYTDKIM TYLFDFPHGP KPGSSHRPWQ SYFDLILVDA RKPLFFGEGT VLRQVDTKTG KLKIGTYTGP LQHGIVYSGG SSDTICDLLG AKGKDILYIG DHIFGDILKS KKRQGWRTFL VIPELAQELH VWTDKSSLFE ELQSLDIFLA ELYKHLDSSS NERPDISSIQ RRIKKVTHDM DMCYGMMGSL FRSGSRQTLF ASQVMRYADL YAASFINLLY YPFSYLFRAA HVLMPHESTV EHTHVDINEM ESPLATRNRT SVDFKDTDYK RHQLTRSISE IKPPNLFPLA PQEITHCHDE DDDEEEEEEE E

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

67.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NT5C2 His Protein

SDS-PAGE: Recombinant Human NT5C2 His Protein [NBP1-72471]

SDS-PAGE: Recombinant Human NT5C2 His Protein [NBP1-72471]

SDS-Page: Recombinant Human NT5C2 Protein [NBP1-72471] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-72471
Formulation 20 mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.1 M NaCl, 1 mM DTT, 0.1 mM PMSF
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NT5C2

NT5C2, also known as NT5B or PNT5, has an essential role in the maintenance of purine/pyrimidine nucleotides. It contains a phosphotransferase active site that catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates. In addition, it regulates the level of inosine monophosphate (IMP) and guanosine monophosphate (GMP) pools within cells via hydrolysis. Recombinant human NT5C2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

5'-nucleotidase (purine), cytosolic type B, 5'-nucleotidase, cytosolic II, cN-II, Cytosolic 5'-nucleotidase II, EC 3.1.3, EC 3.1.3.5, GMP, NT5BIMP-specific 5'-NT, NT5CP, PNT5cytosolic purine 5'-nucleotidase, purine 5' nucleotidase

Gene Symbol

NT5C2

Additional NT5C2 Products

Product Documents for Recombinant Human NT5C2 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human NT5C2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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