Recombinant Human Caspase-7 Protein
R&D Systems, part of Bio-Techne | Catalog # 823-C7
Key Product Details
Product Specifications
Source
Ala24-Asp198 (p20) & Ala207-Gln303 (p11)
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The specific activity is >3300 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 823-C7
Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Sucrose with BSA as a carrier protein. |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 823-C7/CF
Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Sucrose. |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Caspase-7
Caspase-7 (Cysteine-aspartic acid protease 7/Casp7; also CMH-1, ICE-LAP3 and Mch3) is a 32 kDa member of the peptidase C14A/IL-1 beta-converting family of enzymes (1, 2, 3). It is widely expressed, except in brain, and is best known as an integral component of the apoptotic cascade. Caspase-7 is considered to be an executioner caspase, as a downstream mediator of apoptotic-associated proteolysis (2, 3). Upon activation, Caspase-7 is known to utilize a Cys residue to cleave multiple substrates, including PARP, procaspase 6, Gas2 and calpstatin (1). Human procaspase-7 is a 34-36 kDa, 303 amino acid (aa) protein (4, 5, 6). Normally, it is an inactive homodimer (1, 2, 7, 8). But following an upstream signal that activates processing proteases, procaspase-7 undergoes proteolytic cleavage to generate an N-terminal 23 aa propeptide, a 175 aa p20/20 kDa subunit (aa 24-198), and a 105 aa C-terminal p12/12 kDa subunit (5). The p20 and p12 subunits noncovalently heterodimerize, and subsequently associate with another p20/p12 heterodimer to form an active antiparallel homodimer. Additional processing of p20 may remove aa 24‑36 to generate p18, while additional processing of p12 will remove aa 199‑206 to generate p11 (9, 10). Multiple proteases can use Caspase-7 as a substrate, and include caspase-1, -3, -8, and -10, granzyme B, calpain-1 and Caspase-7 itself (3, 6, 9, 11). Caspase-7 is found in both cytosol and nucleus, and possesses a potential KKKK nuclear localization signal between aa 38-41 that likely undergoes sumoylation (9, 12). There are two potential isoform variants, one which shows an alternate start site 33 aa upstream of the standard start site, and a second that shows a 105 aa substitution for aa 149-303. Human and mouse Caspase-7 are 82% aa identical at the amino acid level.
References
- Chowdhury, I. et al. (2008) Comp. Biochem. Physiol. B 151:10.
- Boatright, K.M. and G.S. Salvesen (2003) Curr. Opin. Cell Biol. 15:725.
- Launay, S. et al. (2005) Oncogene 24:5137.
- Juan, T. et al. (1997) Genomics 40:86.
- Fernandez-Alnemri, T. et al. (1995) Cancer Res. 55:6045.
- Fernandez-Alnemri, T. et al. (1996) Proc. Natl. Acad. Sci. USA 93:7464.
- Gao, Z. et al. (2007) J. Biol. Chem. 282:30718.
- Riedl, S.J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:14790.
- Gafni, J. et al. (2009) J. Biol. Chem. July 21 [epub ahead of print].
- Lippke, J.A. et al. (1996) J. Biol. Chem. 271:1825.
- Lamkanfi, M. et al. (2008) Mol. Cell. Proteomics 7:2350.
- Hayashi, N. et al. (2006) Neurosci. Lett. 397:5.
Alternate Names
Gene Symbol
UniProt
Additional Caspase-7 Products
Product Documents for Recombinant Human Caspase-7 Protein
Product Specific Notices for Recombinant Human Caspase-7 Protein
For research use only