Recombinant Human Caspr2 His-tag Protein, CF

Name: Recombinant Human Caspr2 His-tag Protein, CF
Brand: R&D Systems
SKU: 8207-CRB

R&D Systems, part of Bio-Techne | Catalog # 8207-CRB

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Product Datasheet / COA / SDS

Key Product Details

Source:	NS0
Accession #:	Q9UHC6.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Caspr2 His-tag Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human Caspr2 His-tag Protein, CF

Source

Mouse myeloma cell line, NS0-derived human Caspr2 protein
Ala28 - Ala1262, with a C-terminal 6-His tag

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala 28

Predicted Molecular Mass

138 kDa

SDS-PAGE

138 kDa

Activity

Measured by its binding ability in a functional ELISA. Recombinant Human Caspr2 His-tag binds to Recombinant Human Contactin-2/TAG1 Protein (Catalog # 1714-CN) with an ED₅₀ of 0.400-4.00 μg/mL.

Scientific Data Examples for Recombinant Human Caspr2 His-tag Protein, CF

	Recombinant Human Caspr2 His-tag Protein Binding Activity. Measured by its binding ability in a functional ELISA. Recombinant Human Caspr2 His-tag Protein (Catalog # 8207-CRB) binds to Recombinant Human Contactin-2/TAG1 Protein ( 1714-CN) with an ED₅₀ of 0.400-4.00 µg/mL.
	Recombinant Human Caspr2 His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Human Caspr2 His-tag Protein (Catalog # 8207-CRB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 130-150 kDa, under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8207-CRB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in water. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Caspr2

Contact-associated Protein-like 2 (Caspr2) is a type I transmembrane member of the neurexin family of adhesion molecules (1). Human Caspr2 is the vertebrate homolog to Drosophila Neurexin IV, and the human and mouse Caspr2 orthologs share 94% amino acid sequence identity. Human Caspr2 contains two EGF-like domains, one F5/8 type C domain, one fibrinogen C-terminal domain, and four laminin G-like domains. It is highly expressed in neuronal tissue where it is primarily localized to the juxtaparanodal region of the axonal membrane (1, 2). Caspr2 acts in conjunction with 4.1B and Tag-1 as a scaffold that clusters Kv1 potassium channels at the juxtaparanodal region and is critical for axo-glial contacts (1-6). Caspr2 interacts with carboxypeptidase E in Golgi bodies during intracellular trafficking to the cell membrane (7). Caspr2 is also required for dendrite arborization and the normal development of neural networks (8). Mutations in Caspr2 are associated with predisposition to autism spectrum disorders, epilepsy, attention-deficit hyperactive disorder, and schizophrenia (8-11). The presence of autoantibodies against Caspr2 is associated with encephalitis, epilepsy, dysarthria, and paroxysmal kinesigenic dystonia (12-14). R&D Systems in-house testing indicates that Caspr2 can enhance bovine corneal endothelial cell adhesion.

Item	Value
References	Poliak, S. et al. (1999) Neuron 24:1037. Poliak, S. et al. (2001) J. Neurosci. 21:7568. Horresh, I. et al. (2010) J. Neurosci. 30:2480. Traka, M. et al. (2003) J. Cell Biol. 162:1161. Denisenko-Nehrbass, N. et al. (2003) Eur. J. Neurosci. 17:411. Poliak, S. et al. (2003) J. Cell Biol. 162:1149. Oiso, S. et al. (2009) J. Neurochem. 109:158. Anderson, G.R. et al. (2012) Proc. Natl. Acad. Sci. USA 109:18120. Penagarikano, O. and D.H. Geschwind (2012) Trends Mol. Med. 18:156. Friedman, J.I. et al. (2008) Mol. Psychiatry 13:261. Elia, J. et al. (2010) Mol. Psychiatry 15:637. Lancaster, E. et al. (2011) Ann. Neurol. 69:303. Balint, B. et al. (2013) J. Neurol. Sci. 327:73. Krogias, C. et al. (2013) JAMA Neurol. 70:1056.
Long Name	Contactin Associated Protein-like 2
Entrez Gene IDs	26047 (Human); 66797 (Mouse)
Alternate Names	AUTS15, CASPR2DKFZp781D1846, CDFE, CNTNAP2, Caspr2, Cell recognition molecule Caspr2, KIAA0868, NRXN4, NRXN4Caspr2, PTHSL1, contactin associated protein-like 2, contactin-associated protein 2, contactin-associated protein-like 2, homolog of Drosophila neurexin IV

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Recombinant Human Caspr2 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8207-CRB

Key Product Details

Product Specifications for Recombinant Human Caspr2 His-tag Protein, CF

Scientific Data Examples for Recombinant Human Caspr2 His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Caspr2

Customer Reviews for Recombinant Human Caspr2 His-tag Protein, CF

Product Documents for Recombinant Human Caspr2 His-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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