Recombinant Human CD30/TNFRSF8 Fc Chimera Biotin Protein, CF

Name: Recombinant Human CD30/TNFRSF8 Fc Chimera Biotin Protein, CF
Brand: R&D Systems
SKU: BT11155

R&D Systems, part of Bio-Techne | Catalog # BT11155

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Product Datasheet / COA / SDS

Key Product Details

Source:	HEK293
Structure / Form:	Disulfide linked homodimer, Biotinylated via amines
Applications:	Bioactivity

View Full Technical Details for Recombinant Human CD30/TNFRSF8 Fc Chimera Biotin Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human CD30/TNFRSF8 Fc Chimera Biotin Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human CD30/TNFRSF8 protein

Human CD30/TNFRSF8 (Phe19-Lys379) Accession # P28908.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Phe19

Predicted Molecular Mass

65 kDa

SDS-PAGE

65 kDa

Activity

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human CD30/TNFRSF8 Fc Chimera (Catalog # BT11155) binds to Recombinant Human CD30 Ligand/TNFSF8 (Catalog # 1028-CL) with an ED₅₀ of 0.800-16.0 ng/mL.

Scientific Data Examples for Recombinant Human CD30/TNFRSF8 Fc Chimera Biotin Protein, CF

	Biotinylated Recombinant Human CD30/TNFRSF8 Fc Chimera Protein Binding Activity. In a functional ELISA, Biotinylated Recombinant Human CD30/TNFRSF8 Fc Chimera Protein (Catalog # BT11155) binds to Recombinant Human CD30 Ligand/TNFSF8 (1028-CL) with an ED₅₀ of 0.800‑16.0 ng/mL.
	Biotinylated Recombinant Human CD30/TNFRSF8 Fc Chimera Protein SDS-PAGE. 2 μg/lane of Biotinylated Recombinant Human CD30/TNFRSF8 Fc Chimera Protein (Catalog # BT11155) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 80-115 kDa and 160-230 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT11155

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD30/TNFRSF8

CD30, also known as Ki-1 antigen and TNFRSF8, is a 120 kDa type I transmembrane glycoprotein belonging to the TNF receptor superfamily (1, 2). Mature human CD30 consists of a 361 amino acid (aa) extracellular domain (ECD) with six cysteine-rich repeats, a 28 aa transmembrane segment, and a 188 aa cytoplasmic domain (3). In contrast, mouse and rat CD30 lack 90 aa of the ECD and contain only three cysteine-rich repeats. Within common regions of the ECD, human CD30 shares 53% and 49% aa sequence identity with mouse and rat CD30, respectively. Alternate splicing of human CD30 generates an isoform that includes only the C‑terminal 132 aa of the cytoplasmic domain. CD30 is normally expressed on antigen-stimulated Th cells and B cells (4 ‑ 6). However, it is up‑regulated in Hodgkin’s disease (on Reed-Sternberg cells), other lymphomas, chronic inflammation, and autoimmunity (7). CD30 binds to CD30 Ligand/TNFSF8 which is expressed on activated Th cells, monocytes, granulocytes and medullary thymic epithelial cells (1, 5). CD30 signaling co‑stimulates antigen-induced Th0 and Th2 proliferation and cytokine secretion but favors a Th2-biased immune response (8). In the absence of antigenic stimulation, it can still induce T cell expression of IL‑13 (9). CD30 contributes to thymic negative selection by inducing the apoptotic cell death of CD4+CD8+ T cells (10, 11). In B cells, CD30 ligation promotes cellular proliferation and antibody production in addition to the expression of CXCR4, CCL3, and CCL5 (5, 12). An 85 ‑ 90 kDa soluble form of CD30 is shed from the cell surface by TACE-mediated cleavage (13, 14). Soluble CD30 retains the ability to bind CD30 Ligand and functions as an inhibitor of normal CD30 signaling (15).

Item	Value
References	Kennedy, M.K. et al. (2006) Immunology 118:143. Tarkowski, M. (2003) Curr. Opin. Hematol. 10:267. Durkop, H. et al. (1992) Cell 68:421. Hamann, D. et al. (1996) J. Immunol. 156:1387. Shanebeck, S.D. et al. (1995) Eur. J. Immunol. 25:2147. Gruss, H.-J. et al. (1994) Blood 83:2045. Oflzoglu E. et al. (2009) Adv. Exp. Med. Biol. 647:174. Del Prete, G. et al. (1995) J. Exp. Med. 182:1655. Harlin, H. et al. (2002) J. Immunol. 169:2451. Amakawa, R. et al. (1996) Cell 84:551. Chiarle, R. et al. (1999) J. Immunol. 163:194. Vinante, F. et al. (2002) Blood 99:52. Hansen, H.P. et al. (1995) Int. J. Cancer 63:750. Hansen, H.P. et al. (2000) J. Immunol. 165:6703. Hargreaves, P.G. and A. Al-Shamkhani (2002) Eur. J. Immunol. 32:163.
Entrez Gene IDs	943 (Human); 21941 (Mouse); 25069 (Rat)
Alternate Names	CD30, CD30 antigen, CD30KI-1, CD30L receptor, D1S166EKi-1, Ki-1 antigen, Lymphocyte activation antigen CD30, TNFRSF8, cytokine receptor CD30, tumor necrosis factor receptor superfamily member 8, tumor necrosis factor receptor superfamily, member 8

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Recombinant Human CD30/TNFRSF8 Fc Chimera Biotin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT11155

Key Product Details

Product Specifications for Recombinant Human CD30/TNFRSF8 Fc Chimera Biotin Protein, CF

Scientific Data Examples for Recombinant Human CD30/TNFRSF8 Fc Chimera Biotin Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: CD30/TNFRSF8

Customer Reviews for Recombinant Human CD30/TNFRSF8 Fc Chimera Biotin Protein, CF

Product Documents for Recombinant Human CD30/TNFRSF8 Fc Chimera Biotin Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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