Recombinant Human Complement Component C1rLP His Protein, CF

Name: Recombinant Human Complement Component C1rLP His Protein, CF
Brand: R&D Systems
SKU: 11614-CP

R&D Systems, part of Bio-Techne | Catalog # 11614-CP

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Product Datasheet / COA / SDS

HA-tag His-tag

Key Product Details

Source:	CHO
Accession #:	NP_057630.1
Applications:	Binding Activity

View Full Technical Details for Recombinant Human Complement Component C1rLP His Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human Complement Component C1rLP His Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human Complement Component C1rLP protein
Cys36-Asp487 with N-terminal HA (YPYDVPDYA) and 6-His tags

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Tyr

Predicted Molecular Mass

52 kDa

SDS-PAGE

52 kDa

Activity

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >30 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human Complement Component C1rLP His Protein, CF

	Recombinant Human Complement Component C1rLP HA-tag His-tag Protein Enzyme Activity. Recombinant Human Complement Component C1rLP HA-tag His-tag (Catalog # 11614-CP) is measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB).
	Recombinant Human Complement Component C1rLP HA-tag His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Human Complement Component C1rLP HA-tag His-tag Protein (Catalog # 11614-CP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70‑76 kDa, under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11614-CP

Formulation	Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

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Desired Reconstitution Concentration

Background: Complement Component C1rLP

Recombinant human Complement C1r subcomponent-like protein (C1rLP), also known as C1r-like serine protease analog protein (CLSPa) is a 487 residue glycosylated, secreted serine peptidase-like protein (1, 2). It contains an N-terminal signal peptide, a CUB domain for substrate and protein-protein interaction, a truncated complement control protein module, and a trypsin-like serine protease domain with a characteristic catalytic triad but lacks an activation consensus peptide and an EGF domain found in homologous complement cascade proteases like C1r protein (1, 2). It is broadly expressed and secreted in tissues and abundantly found in liver, kidney, and myeloid cells where significant expression of serine proteases typically occurs (1). C1rLP has esterolytic activity on peptide thioesters with arginine at the P1 position but lower catalytic efficiency compared to C1r (2). It was reported to mediate cleavage of haptoglobin (3) and subsequently proposed to be of use in hemoglobin-binding therapeutics (4). Complement cascade serine proteases such as C1r play a role in innate immunity but the role of C1rLP in complement-mediated function is unclear as both activation and inhibition roles have been described in the literature (1, 2). C1rLP has been shown to be a pathological marker in immune microenvironments including within acute myocardial infarction and COVID19 as well as several types of cancer such as colon, leukemia, brain, endometrial, and melanoma (5-12). As a pathological marker, it has also been proposed as a potential therapeutic target for the treatment of these diseases (12).

Item	Value
References	Lin, N. et al. (2004) Biochem. Biophys. Res. Commun. 321:329. Ligoudistianou, C. et al. (2005) Biochem. J. 387:165. Wicher, K.B. et al. (2004) Proc. Natl. Acad. Sci. 101:14390. Schaer, C.A. et al. (2018) BMC Biotechnol. 18:15. Liu, X. et al. (2021) ACS Omega 6:7951. Yang, S. et al. (2022) Front. Oncol. 12:934928. Chen J. et al. (2023) Aging 15:4444. Njoku K. et al. (2023) Br. J. Cancer 128:1723. Yoon, H.G. et al. (2023) PLoS One 18:e0295061. Beck. H.C. et al. (2024) Int. J. Mol. Sci. 25:2638. Liu, G. et al. (2024) Heliyon 11:e32337. Zhang, M. et al. (2024) Heliyon 10:e30616.
Long Name	Complement C1r Subcomponent-like Protein
Entrez Gene IDs	51279 (Human); 232371 (Mouse)
Alternate Names	C1RL, C1RL1, C1RL1complement C1r subcomponent-like protein, C1RLPEC 3.4.21.-, C1r-LPcomplement C1r-like proteinase, C1r-like protein, C1r-like serine protease analog protein, CLSPa, Complement Component C1rLP, EC 3.4.21, EC 3.4.21.41, complement component 1, r subcomponent-like

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Recombinant Human Complement Component C1rLP His Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11614-CP

Key Product Details

Product Specifications for Recombinant Human Complement Component C1rLP His Protein, CF

Scientific Data Examples for Recombinant Human Complement Component C1rLP His Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Complement Component C1rLP

Customer Reviews for Recombinant Human Complement Component C1rLP His Protein, CF

Product Documents for Recombinant Human Complement Component C1rLP His Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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