Recombinant Human DLL4 Avi-tag His-tag Protein, CF

Name: Recombinant Human DLL4 Avi-tag His-tag Protein, CF
Brand: R&D Systems
SKU: AVI1506

R&D Systems, part of Bio-Techne | Catalog # AVI1506

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Product Datasheet / COA / SDS

Key Product Details

Source:	CHO
Accession #:	Q9NR61.1
Structure / Form:	Biotinylated via Avi-tag
Applications:	Bioactivity

View Full Technical Details for Recombinant Human DLL4 Avi-tag His-tag Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human DLL4 Avi-tag His-tag Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human DLL4 protein

Human DLL4 (Ser27-Pro524) Accession # Q9NR61.1	Avi-tag	6-His tag
N-terminus		C-terminus

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ser27

Predicted Molecular Mass

58 kDa

SDS-PAGE

58 kDa

Activity

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human DLL4 Avi-tag His-tag (Catalog # AVI1506) binds to Recombinant Human Notch-1 Fc Chimera (Catalog # 3647-TK) with an ED₅₀ of 0.600-7.20 μg/mL.

Scientific Data Examples for Recombinant Human DLL4 Avi-tag His-tag Protein, CF

	Biotinylated Recombinant Human DLL4 Avi-tag His-tag Protein Binding Activity. In a functional ELISA, Biotinylated Recombinant Human DLL4 Avi-tag His-tag Protein (Catalog # AVI1506) binds to Recombinant Human Notch‑1 Fc Chimera (3647-TK) with an ED₅₀ of 0.600-7.20 μg/mL.
	Biotinylated Recombinant Human DLL4 Avi-tag His-tag Protein SDS-PAGE. 2 μg/lane of Biotinylated Recombinant Human DLL4 Avi-tag His-tag Protein (Catalog # AVI1506) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 63-75 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI1506

Formulation	Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: DLL4

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal regulated intramembrane proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/ gamma secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1). Our Avi-tag Biotinylated Recombinant Human DLL4features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Item	Value
References	Shutter, J.R. et al. (2000) Genes Dev. 14:1313. Iso, Tatsuya et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543. Walker, L. et al. (2001) Stem Cells 19:543. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.
Long Name	Delta-like 4
Entrez Gene IDs	54567 (Human); 54485 (Mouse); 100152163 (Porcine)
Alternate Names	DLL4, Delta 4 precursor, Drosophila Delta homolog 4, MGC126344, delta 4, delta ligand 4, delta-like 4 (Drosophila), delta-like 4 homolog, delta-like 4 homolog (Drosophila), delta-like 4 protein, delta-like protein 4, delta4, hdelta2, notch ligand DLL4, notch ligand delta-2

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Recombinant Human DLL4 Avi-tag His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI1506

Key Product Details

Product Specifications for Recombinant Human DLL4 Avi-tag His-tag Protein, CF

Scientific Data Examples for Recombinant Human DLL4 Avi-tag His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: DLL4

Customer Reviews for Recombinant Human DLL4 Avi-tag His-tag Protein, CF

Product Documents for Recombinant Human DLL4 Avi-tag His-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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