Recombinant Human IgLON5 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9728-IG

R&D Systems, part of Bio-Techne
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9728-IG-050
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Key Product Details

Source

CHO

Accession #

A6NGN9

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IgLON5 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IgLON5 protein
Human IGLON-5
(Leu33-Asn314)
Accession # A6NGN9		 IEGRMD Human IgG1
(Pro100-Lys330)

N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu33

Predicted Molecular Mass

58 kDa 

SDS-PAGE

77-92 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human IgLON5 Fc Chimera, immobilized at 2.5-5 μg/mL, is able to significantly enhance neurite outgrowth.

Formulation, Preparation and Storage

9728-IG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgLON5

IGLON family member 5 (IgLON5) is a member of the IgLON (LSAMP, OPCML/OBCAM, neurotrimin) family of neuronal cell adhesion molecules which, in turn, are part of the much larger Ig domain-containing superfamily (1). The overall structure of IgLON proteins contain three immunoglobulin domains, with numerous potential glycosylation sites, followed by a glycosylphosphatidylinositol (GPI) anchor (2). Mature human IgLON5 is 306 amino acids (aa) in length and shares 98% aa identity with both mouse and rat. Although the specific function of IgLON5 has yet to be elucidated, IgLON proteins are known to form homophilic and heterophilic complexes along the cell surface and several have been shown to shed from mature cortical neurons in a metalloproteinase-dependent manner. These go on to play a role in processes such as neuronal differentiation and survival, axon guidance and outgrowth and cell migration (3, 4). IgLON-5 is expressed in both neurons and oligodendrocytes (5). In the brain, the level of Iglon5 transcript is highest in the brainstem, thalamus and eye, and weakest in the hippocampus, septum, and pituitary gland (6). IgLON-5 is also expressed in non-neural organs and tissue types where it may function as a tumor-suppressor (6-7). Newly classified anti-IgLON5 syndrome is a novel neuronal tauopathy where antibodies target surface IgLON5, causing an irreversible internalization of the protein, manifesting as sleep dysfunction (8-10).

References

  1. Sellar G, et al. (2003) Nat. Genet. 34(3):337.
  2. Itoh S, et al. (2008) Biochemistry 47(38):10132.
  3. Sanz R, et al. (2015) J. Biol. Chem. 290(7):4330.
  4. Karagogeos D (2003) Front. Biosci. 8:1304.
  5. Sharma, K. et. al. (2015) Nat. Neurosci. 18:1819.
  6. Vanaveski, T. et. al. (2017) Front. Neurosci. 11:Article 38.
  7. Baroy, T. et. al. (2014) Mol. Cancer 13:139.
  8. Sabater L, et al. (2014) Lancet Neurol. 13(6):575.
  9. Sabater L, et al. (2016) J. Neuroinflamm. 13:226.
  10. Gelpi E, et al. (2016) Acta Neuropathol. 132:531.

Long Name

IgLON Family Member 5

Alternate Names

hCG1651476, Ig-like domain-containing protein ENSP00000270642, igLON family member 5

Entrez Gene IDs

402665 (Human); 210094 (Mouse); 308557 (Rat)

Gene Symbol

IGLON5

UniProt

A6NGN9

Additional IgLON5 Products

Product Documents for Recombinant Human IgLON5 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IgLON5 Fc Chimera Protein, CF

For research use only

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