Recombinant Human IL-2 (CD122-Directed) Protein, CF

Name: Recombinant Human IL-2 (CD122-Directed) Protein, CF
Brand: R&D Systems
SKU: BT-002DBR

R&D Systems, part of Bio-Techne | Catalog # BT-002DBR

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AI Modified Protein

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Key Product Details

Il-2 receptor engagement can impact the growth and phenotype of cultured immune cells such as T, NK, or Tumor Infiltrating T cells. By engaging the beta receptor (CD122) with higher affinity, continued proliferation of T cells has been observed. Utilizing generative AI to develop an optimized IL-2 cytokine with enhanced beta receptor (CD122) engagement offers superior performance while maintaining greater than 95% homology to its wild-type counterpart including:

Enhanced CD122 Binding: Increased affinity to the IL-2 beta receptor (CD122) with decreased affinity to IL-2 alpha receptor (CD25), allowing proliferation of effector T cells
allowing proliferation of effector T cells
Improved Output: Maintains growth of CD8⁺/CD4⁺ T cells for workflows aimed at extending proliferation of T cells.
Sustained Phenotype: Improved proliferation capacity without increased exhaustion nor more terminally differentiated T cells.

Source:	E. coli
Accession #:	P60568.1
Applications:	Bioactivity

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Technical Data
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Product Specifications for Recombinant Human IL-2 (CD122-Directed) Protein, CF

Source

E. coli-derived human IL-2 protein
Ala21-Thr153 (C145S) with and without an N-terminal Met
Proprietary point mutations

Endotoxin level

<0.20 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala21 & Met-Ala21

Predicted Molecular Mass

15 kDa

SDS-PAGE

15 kDa

Activity

Measured in a cell proliferation assay using NK-92 human natural killer lymphoma cells. The ED₅₀ for this effect is 0.0500-0.500 ng/mL.

Scientific Data Examples for Recombinant Human IL-2 (CD122-Directed) Protein, CF

	Recombinant Human IL-2 (CD122-Directed) Protein Bioactivity. Recombinant Human IL-2 (CD122-Directed) Protein (Catalog # BT-002DBR) stimulates proliferation of NK-92 human natural killer lymphoma cells. The ED₅₀ for this effect is 0.0500-0.500 ng/mL.
	Recombinant Human IL-2 (CD122-Directed) Protein SDS-PAGE. 2 μg/lane of Recombinant Human IL-2 (CD122-Directed) Protein (Catalog # BT-002DBR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 12‑14 kDa, under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT-002DBR

Formulation	Lyophilized from a 0.2 μm filtered solution in Sodium Acetate with Trehalose.
Reconstitution	Reconstitute the 10 μg size at 100 μg/mL in water. Reconstitute all the other sizes at 500 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-2

CD122 directed IL-2 has a 7-fold increased binding affinity to the human IL-2 beta receptor while exhibiting significantly reduced affinity for the IL-2 alpha receptor. According to research by Levin (2012), this configuration results in more effective expansion of effector cells and less Treg (1). Our findings show that CD122 directed IL-2 improves immune cell expansion for workflows requiring significant amounts of IL-2. One concern with improving T cell expansion is the possibility of increasing the number of terminally differentiated and exhausted T cells with reduced anti-tumor activity. However, our studies have shown that CD122 directed IL-2 significantly improves T cell expansion without changing T cell phenotype or the expression of exhaustion markers.

Item	Value
References	Levin, A., Bates, D., Ring, A. et al. (2012) Nature. 484:529.
Long Name	Interleukin 2
Entrez Gene IDs	3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names	Aldesleukin, IL-2, IL-2lymphokine, IL2, Proleukin, T cell growth factor, T-cell growth factor, TCGF, interleukin 2, interleukin-2, involved in regulation of T-cell clonal expansion

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Recombinant Human IL-2 (CD122-Directed) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT-002DBR

Key Product Details

Product Specifications for Recombinant Human IL-2 (CD122-Directed) Protein, CF

Scientific Data Examples for Recombinant Human IL-2 (CD122-Directed) Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: IL-2

Customer Reviews for Recombinant Human IL-2 (CD122-Directed) Protein, CF

Product Documents for Recombinant Human IL-2 (CD122-Directed) Protein, CF

Product Datasheets

COA

Certificate of Analysis

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