Recombinant Human KGF/FGF-7 Protein, CF

Name: Recombinant Human KGF/FGF-7 Protein, CF
Brand: R&D Systems
SKU: BT-KGF

R&D Systems, part of Bio-Techne | Catalog # BT-KGF

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Product Datasheet / COA / SDS

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Qty

Key Product Details

Source:	E. coli
Accession #:	P21781.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human KGF/FGF-7 Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human KGF/FGF-7 Protein, CF

Source

E. coli-derived human KGF/FGF-7 protein
Cys32-Thr194, with an N-terminal Met

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Cys32 & Asn33

Predicted Molecular Mass

19 kDa

SDS-PAGE

19 kDa

Activity

Measured in a cell proliferation assay using Ba/F3 mouse pro B cells transfected with human FGF RIIb. The ED₅₀ for this effect is 6.00-60.0 ng/mL.

Scientific Data Examples for Recombinant Human KGF/FGF-7 Protein, CF

	Recombinant Human KGF/FGF‑7 Protein Bioactivity. Recombinant Human KGF/FGF‑7 Protein (Catalog # BT-KGF) induced cell proliferation in Ba/F3 mouse pro-B cells transfected with human FGF RIIb. The ED₅₀ for this effect is 6.00-60.0 ng/mL.
	Recombinant Human KGF/FGF‑7 Protein SDS-PAGE. 2 μg/lane of Recombinant Human KGF/FGF‑7 Protein (Catalog # BT-KGF) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 20 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT-KGF

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄ and EDTA with Trehalose.
Reconstitution	Reconstitute the 20 μg size at 200 μg/mL in water. Reconstitute all other sizes at 500 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KGF/FGF-7

Keratinocyte Growth Factor (KGF), or Fibroblast Growth Factor-7 (FGF-7), is an important component in many cell culture protocols. It is widely used to promote the growth and differentiation of epithelial cells, including skin keratinocytes, and lung, corneal, and intestinal epithelia. It is a part of tissue engineering and organoid culture protocols, where it can enhance the generation of complex tissues such as liver, gastrointestinal tract, lung, and mammary gland. Furthermore, KGF/FGF-7 has been shown to stimulate the proliferation of pancreatic endocrine progenitor cells and promote their differentiation into mature insulin-secreting beta cells. In these capacities KGF/FGF-7 has significant clinical relevance in studies to better understand gastrointestinal disorders, wound healing, respiratory disease, and diabetes.

Item	Value
Long Name	Keratinocyte Growth Factor
Entrez Gene IDs	2252 (Human); 403915 (Canine)
Alternate Names	FGF-7, FGF7, HBGF-7, HBGF7, Heparin-binding growth factor 7, KGF, KGFfibroblast growth factor 7 (keratinocyte growth factor), fibroblast growth factor 7HBGF-7, keratinocyte growth factor

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Recombinant Human KGF/FGF-7 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT-KGF

Key Product Details

Product Specifications for Recombinant Human KGF/FGF-7 Protein, CF

Scientific Data Examples for Recombinant Human KGF/FGF-7 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: KGF/FGF-7

Customer Reviews for Recombinant Human KGF/FGF-7 Protein, CF

Product Documents for Recombinant Human KGF/FGF-7 Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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