Recombinant Human LIPA HA-tag His-tag Protein, CF

Name: Recombinant Human LIPA HA-tag His-tag Protein, CF
Brand: R&D Systems
SKU: 11633-LA

R&D Systems, part of Bio-Techne | Catalog # 11633-LA

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Product Datasheet / COA / SDS

Key Product Details

Source:	CHO
Accession #:	P38571.2
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human LIPA HA-tag His-tag Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human LIPA HA-tag His-tag Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human LIPA protein
Gly24-Gln399 with N-terminal HA (YPYDVPDYA) and 6-His tags

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Tyr

Predicted Molecular Mass

45 kDa

SDS-PAGE

45 kDa

Activity

Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl oleate (4-MUO).The specific activity is >7000 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human LIPA HA-tag His-tag Protein, CF

	Recombinant Human LIPA HA-tag His-tag Enzyme Activity. Recombinant Human LIPA HA-tag His-tag (Catalog # 11633-LA) is measured by its ability to cleave a fluorogenic peptide substrate, 4-Methylumbelliferyl oleate (4-MUO).
	Recombinant Human LIPA HA-tag His-tag SDS-PAGE. 2 μg/lane of Recombinant Human LIPA HA-tag His-tag (Catalog # 11633-LA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-67 kDa, under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11633-LA

Formulation	Supplied as a 0.2 μm filtered solution in MES, NaCl and TCEP.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: LIPA

Recombinant human Lysosomal acid lipase (LAL) or Lipase A (LIPA), also known as acid cholesteryl ester hydrolase, is a 378 residue mature, glycosylated, lysosomal serine hydrolase of a family of three mammalian acid lipases. Mature LIPA contains a globular core domain typical of a/b hydrolase-fold family members and a cap domain. Beneath the cap region, the core domain contains the catalytic triad surrounded by a hydrophobic surface that promotes lipid substrate binding (1). Except for erythrocytes, LIPA is broadly expressed in cells (1) and is found in the lysozyme where it is required for hydrolysis of cholesteryl esters and triglycerides from low density lipoproteins (LDL) to generate fatty acids and cholesterol (2). LIPA deficiency from over 40 reported loss-of-function mutations leads to accumulation of cholesterol and triglyceride in the lysosome with resulting dysfunctional cholesterol homeostasis (1, 3). Lysosomal acid deficiency (LAL-D) is an autosomal recessive lysosomal storage disease with two phenotypes including early-onset Wolman disease (WD) and later-onset cholesteryl ester storage disease (CESD) (3, 4). Supplementation of LIPA through enzyme replacement therapy has been shown to be effective and is the standard therapy for LAL-deficiency although additional treatments are being explored (3, 4-6). Due to its central role in lipid regulation, altered function and variants of LIPA have also been implicated in development of atherosclerotic disease and coronary artery disease (1, 3, 7, 8). LIPA has additionally been reported as a therapeutic target for cancer and solid tumors through its role in induction of ER stress (9), its association with specific binding partner of interest (10), and its ability to modulate lipid metabolism in cancer cells (11).

Item	Value
References	Rajamohan, F. et al. (2020) J. Lipid Res. 61:1192. Anderson, R.A. and G.N. Sando (1991) J. Biol. Chem. 266:22479. Maciejko, J.J. (2017) Am. J. Cardiovasc. Drugs 17:217. Korbelius, M. et al. (2023) Trends Mol. Med. 29:425. Burton, B.K. et al. (2015) N. Engl. J. Med. 373:1010. Rader, D.J. (2015) N. Engl. J. Med. 373:1071. Wild, P.S. et al. (2011) Circ. Cardiovasc. Genet. 4:403. Li, F. and H. Zhang (2019) Arterioscler. Thromb. Vasc. Biol. 39:850. Liu, X. et al. (2022) Nat. Cancer 3:866. Collier, A.B. (2024) Cancers 16:500. Jin, Z. et al. (2024) Cancer Res. Commun. 4:2242.
Long Name	Lipase A
Entrez Gene IDs	3988 (Human)
Alternate Names	Acid cholesteryl ester hydrolase, CESD, Cholesteryl esterase, EC 3.1.1, EC 3.1.1.13, LALCESDcholesterol ester hydrolase, LIPA, Lipase A, Sterol esterase, lipase A, lysosomal acid, cholesterol esterase, lysosomal acid lipase, lysosomal acid lipase/cholesteryl ester hydrolase

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Recombinant Human LIPA HA-tag His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11633-LA

Key Product Details

Product Specifications for Recombinant Human LIPA HA-tag His-tag Protein, CF

Scientific Data Examples for Recombinant Human LIPA HA-tag His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: LIPA

Customer Reviews for Recombinant Human LIPA HA-tag His-tag Protein, CF

Product Documents for Recombinant Human LIPA HA-tag His-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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