Recombinant Human MDH1 His-tag Protein, CF

Name: Recombinant Human MDH1 His-tag Protein, CF
Brand: R&D Systems
SKU: 11632-MH

R&D Systems, part of Bio-Techne | Catalog # 11632-MH

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Product Datasheet / COA / SDS

Key Product Details

Source:	E. coli
Accession #:	P40925.4
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human MDH1 His-tag Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human MDH1 His-tag Protein, CF

Source

E. coli-derived human MDH1 protein
Met1-Ala334, with a C-terminal 6-His tag

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Met1 & Ser2

Predicted Molecular Mass

37 kDa

SDS-PAGE

37 kDa

Activity

Measured by its ability to produce malate from oxaloacetate.

The specific activity is >55,000 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human MDH1 His-tag Protein, CF

	Recombinant Human MDH1 His-tag Enzyme Activity. Recombinant Human MDH1 His-tag (Catalog # 11632-MH) is measured by its ability to produce malate from oxaloacetate.
	Recombinant Human MDH1 His-tag SDS-PAGE. 2 μg/lane of Recombinant Human MDH1 His-tag (Catalog # 11632-MH) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-38 kDa, under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11632-MH

Formulation	Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MDH1

Malate Dehydrogenase 1 (MDH1), also known as aromatic alpha-keto acid reductase, belongs to the family of nicotinamide adenine dinucleotide (NAD)-dependent dehydrogenases and is a key enzyme in the malate-aspartate shuttle where it plays a critical role in the regulation of metabolic activity in humans (1, 2). MDH1 is an active homodimer of monomers that each contain the characteristic conserved Rossman fold, similar NAD binding sites, and dimeric quaternary structure of MDH enzymes (3). MDH is ubiquitously expressed in cells in two separate isoforms that differ in cellular localization and share only 26% sequence homology (3). While MDH2 is a mitochondrial form involved in the regulation of mitochondrial NAD levels within the citric acid cycle, MDH1, the cytosolic form, plays an important role in the regulation of cytosolic NAD levels. Increased cytosolic NAD levels are necessary for maintaining enhanced glycolysis of proliferating cancer cells (4) and MDH1 is overexpressed in tumors and correlated with poor prognosis (5-8). In addition, MDH1 has been found to serve as a potential marker in several diseases with inflammation and deficiency of MDH1 is associated with early onset severe encephalopathy and associated with acute liver failure (2, 9-11) making it a metabolic therapeutic target of interest with several potential applications (1, 3, 6, 12, 13).

Item	Value
References	Godesi, S. et al. (2023) Pharmaceuticals 16:683. Khamis, A.A. et al. (2024). Genes Nutr. 19:20. McCue, W.M and B.C Finzel (2022) ASC Omega 7:207. Lunt, S.Y. et al. (2011) Annu. Rev. Cell Dev. Biol. 27:441. Wang, Y.P. et al. (2016) Mol. Cell. 64:673. Zhang, B. et al. (2017) J. Cancer 8:2088. Sun, W. et al. (2024) BMC Cancer. 24:905. Wang, J. et al. (2024) Clin. Transl. Med. 14:e1680. Broeks, M.H. et al. (2019) Hum. Genet. 138:1247. Shi, C. et al. (2024) iScience. 27:109678. Zeng, X. et al. (2024) Mol. Neurodegener. 19:68. Hanse, E. et al. (2017) Oncogene 36:3915. Naik, R. et al. (2017) J. Med. Chem. 60:8631.
Long Name	malate dehydrogenase 1
Entrez Gene IDs	4190 (Human); 17449 (Mouse); 24551 (Rat)
Alternate Names	Cytosolic malate dehydrogenase, DEE88, Diiodophenylpyruvate reductase, EC 1.1.1.37, EC 1.1.1.96, EIEE88, HEL-S-32, KAR, MDH-s, MDH1, MDHA, MGC:1375, MOR2, Malate dehydrogenase, cytoplasmic, aromatic alpha-keto acid reductase, cytoplasmic, epididymis secretory protein Li 32, malate dehydrogenase 1, NAD (soluble), malate dehydrogenase, cytoplasmic; malate dehydrogenase, peroxisomal, soluble malate dehydrogenase

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Recombinant Human MDH1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11632-MH

Key Product Details

Product Specifications for Recombinant Human MDH1 His-tag Protein, CF

Scientific Data Examples for Recombinant Human MDH1 His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: MDH1

Customer Reviews for Recombinant Human MDH1 His-tag Protein, CF

Product Documents for Recombinant Human MDH1 His-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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