Recombinant Human MMP-9 Activated Protein, CF

Name: Recombinant Human MMP-9 Activated Protein, CF
Brand: R&D Systems
SKU: 11602-MP

R&D Systems, part of Bio-Techne | Catalog # 11602-MP

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Product Datasheet / COA / SDS

Key Product Details

Source:	CHO
Accession #:	P14780.2
Structure / Form:	Activated
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human MMP-9 Activated Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human MMP-9 Activated Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human MMP-9 protein
Ala20-Asp707 (Gln279Arg)
The proform was activated.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Phe107 & Gly547

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH₂ (Catalog # ES001). The specific activity is >1000 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human MMP-9 Activated Protein, CF

	Recombinant Human MMP‑9 Activated Protein Enzyme Activity. Recombinant Human MMP-9 Activated (Catalog # 11602-MP) is measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (ES001).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11602-MP

Formulation	Supplied as a 0.2 μm filtered solution in Tris, CaCl₂, NaCl and Brij-35.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-9

Recombinant human Active Matrix Metalloproteinase 9 (MMP-9), also known as gelatinase B, is a member of the MMP family of zinc and calcium-dependent endopeptidases. MMP-9 protein is synthesized as a pre-proenzyme in specific cells such as neutrophils, macrophages, fibroblasts and endothelial cells (1). Expressed MMP-9 contains a signal peptide to transport it to the extracellular matrix (ECM), a hinge region, a propeptide region, a catalytic domain, and a hemopexin-like domain that is important for substrate recognition (2, 3). In addition to having three fibronectin type II domains that contribute to substrate binding and an active site, the catalytic domain of MMP-9 also contains a zinc-binding region that interacts with a cysteine in the propeptide to maintain latency. Consequently, removal of the propeptide through cleavage by proteases in the ECM, such as MMP-3, activates the protein (2, 3). MMP-9 has specificity for targets containing an established preferred consensus sequence (3-5) and has a broad range of substrates within the ECM including gelatin, collagen, elastin that contributes to its role in ECM remodeling and extracellular domain cell surface protein release from the plasma membrane (3). Due to its activity in the ECM, MMP-9 plays a role in many biological processes and can serve as a biomarker in tumor invasion and mestastasis (3, 6) of many cancers including colon, ovarian, breast, osteosarcoma, and lung cancers (7-11) making it a therapeutic target (6, 8, 12). MMP-9 modeling of the ECM also leads to a pivotal role in other inflammation- and autoimmune-related diseases (3, 13, 14).

Item	Value
References	Vandooren, J. et al. (2013) Crit. Rev. Biochem. Mol. Biol. 48: 222. Roeb, E. et al. (2002) J. Biol. Chem. 277: 50326. Huang, H. (2018) Sensors. 18:3249. Kridel, S.J. et al. (2001) J. Biol. Chem. 276: 20572. Prudova, A. et al. (2010) Mol. Cell Proteom. 9:894. Kalali, D. (2023) Glob. Med. Genet. 10:48. Hu, X. et al. (2012) Arch. Glynecol. Obstet. 286:1537. Wang, J. et al. (2014) Clin. Chim. Acta. 433:225. Blanco-Prieto, S. et al. (2017) BMC Cancer 17:823. Liang, S. and L. Chang (2018) Biomark. Med. 12:393. Malik, S. et al. (2024) Sci. Rep. 14:15117. Roy, R. et al. (2009) J. Clin. Oncol. 27:5287. Ram, M. et al. (2006) J. Clin. Immunol. 26:299. Kim, I.S. et al. (2023) Curr. Med. Chem. 30:2075.
Long Name	Matrix Metalloproteinase 9
Entrez Gene IDs	4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)
Alternate Names	92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, EC 3.4.24, EC 3.4.24.35, GELB, Gelatinase B, MANDP2, MMP-9, MMP9, macrophage gelatinase, matrix metallopeptidase 9, matrix metalloproteinase 9, matrix metalloproteinase-9, type V collagenase

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Recombinant Human MMP-9 Activated Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11602-MP

Key Product Details

Product Specifications for Recombinant Human MMP-9 Activated Protein, CF

Scientific Data Examples for Recombinant Human MMP-9 Activated Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: MMP-9

Customer Reviews for Recombinant Human MMP-9 Activated Protein, CF

Product Documents for Recombinant Human MMP-9 Activated Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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