Recombinant Human N-Acetylglucosaminyltransferase 3/MGAT3 CF

R&D Systems, part of Bio-Techne | Catalog # 7359-GT

R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Fluorescent Glycan Labeling and Detection

Source

CHO

Accession #

Q09327

Conjugate

Unconjugated

Applications

Enzyme Activity

View all N-Acetylglucosaminyltransferase III/MGAT3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human N-Acetylglucosaminyltransferase III/MGAT3 protein
Tyr31-Val533, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr31

Predicted Molecular Mass

58 kDa

SDS-PAGE

42-54 & 64-70 kDa, reducing conditions

Activity

Measured by its ability to transfer N-Acetyl-alpha -D-glucosamine from UDP-N-Acetyl-alpha -D-glucosamine to a biantennary N-linked core pentasaccharide in a CD39L3 coupled assay.
The specific activity is >100 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7359-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: N-Acetylglucosaminyltransferase III/MGAT3

Mannosylglycoprotein N-acetyl-glucosaminyltransferase 3 (MGAT3), also known as GnT-III, regulates the branching of N-glycans. By transferring a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides MGAT3 produces a bisecting GlcNAc structure (1). Bisecting GlcNAc is involved in a number of biological events, including the suppression of metastasis of cancer cells through modification on integrins (2, 3, 4) and the reduction of hepatitis B virus replication in hepatoma cells (5). The effect of the bisecting GlcNAc on a cancer cell counteracts that of the beta1,6-GlcNAc created by MGAT5 (4, 6). Although MGAT3 and MGAT5 are functionally related, there is no significant homology at their primary sequences. In addition, bisecting GlcNAc on human Ig antibodies enhances their effector functions (7). Transgenic mice over expressing MGAT3 had aberrant glycosylation on apolipoprotein B and developed fatty livers (8). The enzymatic activity of recombinant human MGAT3 was determined using a phosphatase-coupled glycosyltransferase assay (9).

References

  1. Ihara, Y. et al. (1993) J. Biochem. 113:692.
  2. Yoshimura, M. et al. (1995) Proc. Natl. Acad. Sci. USA 92:8754.
  3. Isaji T, et al. (2004) J. Biol. Chem. 279:19747.
  4. Sato, Y. et al. (2009) J. Biol. Chem. 284:11873.
  5. Miyoshi, E. et al. (1995) J. Biol. Chem. 270:28311.
  6. Gu, J. and Taniguchi, N. (2008) Cell Adh. Migr. 2:243.
  7. Stanley P. (2002) Biochim. Biophys. Acta. 1573:363.
  8. Ihara, Y. et al. (1998) Proc. Natl. Acad. Sci. USA 95:2526.
  9. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

beta-1,4-Mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

Alternate Names

GGNT3, GNT-III, GNT3

Entrez Gene IDs

4248 (Human); 17309 (Mouse); 29582 (Rat)

Gene Symbol

MGAT3

UniProt

Q09327 (Human)

Additional N-Acetylglucosaminyltransferase III/MGAT3 Products

Product Documents for Recombinant Human N-Acetylglucosaminyltransferase 3/MGAT3 CF

Product Datasheets

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Product Specific Notices for Recombinant Human N-Acetylglucosaminyltransferase 3/MGAT3 CF

For research use only

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