Recombinant Human OX40/TNFRSF4 Fc Avi-tag Protein, CF

Name: Recombinant Human OX40/TNFRSF4 Fc Avi-tag Protein, CF
Brand: R&D Systems
SKU: AVI10842

R&D Systems, part of Bio-Techne | Catalog # AVI10842

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Product Datasheet / COA / SDS

Biotinylated

Key Product Details

Source:	HEK293
Accession #:	P43489.1
Structure / Form:	Disulfide-linked homodimer, biotinylated via Avi-tag
Applications:	Bioactivity

View Full Technical Details for Recombinant Human OX40/TNFRSF4 Fc Avi-tag Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human OX40/TNFRSF4 Fc Avi-tag Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human OX40/TNFRSF4 protein

Human OX40/TNFRSF4 (Leu29-Ala216) Accession # P43489.1	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu29

Predicted Molecular Mass

49 kDa

SDS-PAGE

49 kDa

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human OX40 Ligand/TNFSF4 (Catalog # 1054-OX) is immobilized at 0.500 µg/mL (100 µL/well), Biotinylated Recombinant Human OX40/TNFRSF4 Fc Chimera Avi-tag binds with an ED₅₀ of 3.00-15.0 ng/mL. The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.

Scientific Data Examples for Recombinant Human OX40/TNFRSF4 Fc Avi-tag Protein, CF

	Biotinylated Recombinant Human OX40/TNFRSF4 Fc Chimera Avi-tag Protein Binding Activity. When Recombinant Human OX40 Ligand/TNFSF4 (1054-OX) is immobilized at 0.500 μg/mL (100 μL/well), Biotinylated Recombinant Human OX40/TNFRSF4 Fc Chimera Avi-tag (Catalog # AVI10842) binds with an ED50 of 3.00-15.0 ng/mL.
	Biotinylated Recombinant Human OX40/TNFRSF4 Fc Chimera Avi-tag Protein SDS-PAGE. 2 μg/lane of Biotinylated Recombinant Human OX40/TNFRSF4 Fc Chimera Avi-tag Protein (Catalog # AVI10842) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 64-72 kDa and 128-144kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10842

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: OX40

OX40 (CD134; TNFRSF4) is a T cell co-stimulatory molecule of the TNF receptor superfamily that coordinates with other co-stimulators (CD28, CD40, CD30, CD27 and 4-1BB) to manage the activation of the immune response (1-3). Human OX40 is a 48 kDa type I transmembrane glycoprotein with a 28 amino acid (aa) signal sequence, a 185 aa extracellular domain (ECD) that contains a cysteine-rich region, a 20 aa transmembrane segment, and a 41 aa cytoplasmic domain (4). The ECD of human OX40 shares 63% sequence identity with the ECD of mouse and rat OX40. OX40 is up-regulated on CD4+ and CD8+ T cells upon engagement of the TCR by antigen presenting cells along with co-stimulation by CD40-CD40 Ligand and CD28-B7 (5, 6). OX40 Ligand is primarily expressed on antigen presenting cells (5). OX40 Ligand engagement of OX40 on activated CD4+ T cells results in increased T cell survival, proliferation, and cytokine production. It also inhibits the conversion of effector T cells into immunosuppressive regulatory T cells (Tregs) and can promote the maintenance of and recall response in memory T cells (3, 7-10). OX40 is constitutively expressed on Tregs and enhances the sensitivity of Tregs to IL-2, thus promoting Treg proliferation. OX40 has also been shown to decrease the cells' immunosuppressive activity on effector T cells (11-14). OX40-OX40 Ligand signaling is involved in allergic airway inflammation, graft-versus-host disease and autoimmune disease (6, 15, 16). Mutations in OX40 and OX40 Ligand are associated with cardiovascular disease (17, 18). Our Avi-tag Biotinylated human OX40/TNFRSF4 Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Item	Value
References	Hori, T. (2006) Int. J. Hematol. 83:17. Latza, U. et al. (1994) Eur. J. Immunol. 24:677. Salek-Ardakani, S. et al. (2003) J. Exp. Med. 198:315. al-Shamkhani, A. et al. (1996) Eur. J. Immunol. 26:1695. Moran, A.E. et al. (2013) Curr. Opin. Immunol. 25:230. Gramaglia, I. et al. (1998) J. Immunol. 161:6510. Xiao, X. et al. (2008) J. Immunol. 181:3193. So, T. and M. Croft (2007) J. Immunol. 179:1427. Mousavi, S.F. et al. (2008) J. Immunol. 181:5990. Bansal-Pakala, P. et al. (2001) Nat. Med. 7:907. Piconese, S. et al. (2010) Eur. J. Immunol. 40:2902. Griseri, T. et al. (2010) J. Exp. Med. 207:699. Xiao, X. et al. (2012) J. Immunol. 188:892. Vu, M.D. et al. (2007) Blood 110:2501. Damayanti, T. et al. (2010) Am. J. Respir. Crit. Care Med. 181:688. Xiao, X. et al. (2012) Nat. Immunol. 13:981. Nakano, M. et al. (2010) Cardiovasc. Res. 88:539. Ishii, N. et al. (2010) Adv. Immunol. 105:63. Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757.
Entrez Gene IDs	7293 (Human); 22163 (Mouse); 25572 (Rat); 102145978 (Cynomolgus Monkey)
Alternate Names	ACT-135, ACT35 antigen, ACT35ATC35 antigen, CD134, CD134 antigen, Ly-70, OX40, OX40 cell surface antigen, OX40 homologue, OX40L receptor, OX40lymphoid activation antigene ACT35, TAX transcriptionally-activated glycoprotein 1 receptor, TNFRSF4, TXGP1L, TXGP1LOX40 antigen, Txgp1, tax-transcriptionally activated glycoprotein 1 receptor, tumor necrosis factor receptor superfamily member 4, tumor necrosis factor receptor superfamily, member 4

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Recombinant Human OX40/TNFRSF4 Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10842

Key Product Details

Product Specifications for Recombinant Human OX40/TNFRSF4 Fc Avi-tag Protein, CF

Scientific Data Examples for Recombinant Human OX40/TNFRSF4 Fc Avi-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: OX40

Customer Reviews for Recombinant Human OX40/TNFRSF4 Fc Avi-tag Protein, CF

Product Documents for Recombinant Human OX40/TNFRSF4 Fc Avi-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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