Recombinant Human TRAIL R2 Fc Alexa Fluor® 647 Protein

Name: Recombinant Human TRAIL R2 Fc Alexa Fluor® 647 Protein
Brand: R&D Systems
SKU: AFR10140

R&D Systems, part of Bio-Techne | Catalog # AFR10140

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Product Datasheet / COA / SDS

Fc Chimera

Key Product Details

Source:	CHO
Accession #:	AAH01281.1
Structure / Form:	Disulfide-linked homodimer Labeled with Alexa Fluor® 647 Excitation Wavelength: 650 nm EmissionWavelength: 668 nm
Applications:	Bioactivity

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Technical Data
Citations / Reviews

Product Specifications for Recombinant Human TRAIL R2 Fc Alexa Fluor® 647 Protein

Source

Chinese Hamster Ovary cell line, CHO-derived human TRAIL R2/TNFRSF10B protein

Human TRAIL R2/TNFRSF10B (Ala54-Ser210) Accession # AAH01281.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala54

Predicted Molecular Mass

44 kDa

SDS-PAGE

44 kDa

Activity

Measured by flow cytometry for its ability to bind anti-human TRAIL R2 Antibody conjugated beads. The concentration of Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Alexa Fluor® 647 that produces 50% of the binding response is 0.300-3.00 ng/mL.

Scientific Data Examples for Recombinant Human TRAIL R2 Fc Alexa Fluor® 647 Protein

	Flow cytometry analysis for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Alexa Fluor® 647 staining on anti-human TRAILR2 Antibody conjugated beads. Streptavidin coated beads conjugated to biotinylated anti-human TRAILR2 Antibody were stained with the indicated concentrations of Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Alexa Fluor® 647 (Catalog # AFR10140).
	Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Alexa Fluor® 647 Protein SDS-PAGE. 2 μg/lane of Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Alexa Fluor® 647 Protein (Catalog # AFR10140) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-61 kDa and 110-120 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

AFR10140

Formulation	Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRAIL R2/TNFRSF10B

Human TRAIL R2, also called DR5 and TRICK 2 is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand) (1). In the new TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% amino acid sequence identity. There are no known rat or mouse homologs to TRAILR2. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis (1-2). The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis (1-2). Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported (3).

Item	Value
References	Chaudhary, P.M. et al. (1997) Immunity 7:821. Walczak, H. et al. (1997) EMBO J. 16:5386. Golstein, P. (1997) Curr. Biol. 7:R750.
Long Name	TRAIL Receptor 2
Entrez Gene IDs	8795 (Human); 21933 (Mouse)
Alternate Names	CD262, CD262 antigen, DR5, DR5TRICK2B, Death receptor 5, Fas-like protein, KILLER/DR5, KILLERapoptosis inducing protein TRICK2A/2B, TNF-related apoptosis-inducing ligand receptor 2, TNFRSF10B, TRAIL R2, TRAIL receptor 2, TRAIL-R2apoptosis inducing receptor TRAIL-R2, TRAILR2, TRAILR2cytotoxic TRAIL receptor-2, TRICK2, TRICK2A, TRICKB, ZTNFR9, death domain containing receptor for TRAIL/Apo-2L, tumor necrosis factor receptor superfamily member 10B, tumor necrosis factor receptor superfamily, member 10b, tumor necrosis factor receptor-like protein ZTNFR9

Product Specific Notices for Recombinant Human TRAIL R2 Fc Alexa Fluor® 647 Protein

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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