Recombinant Mouse EphB3 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 432-B3

R&D Systems, part of Bio-Techne
Discontinued Product
432-B3 has been discontinued. View all EphB3 products.

Key Product Details

Source

NS0

Accession #

P54754

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all EphB3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse EphB3 protein
Mouse EphB3
(Leu30-Thr537)
Accession # P54754		 DIEGRMD Human IgG1

(Pro100-Lys330)

 HHHHHH
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu30

Predicted Molecular Mass

82.8 kDa (monomer)

SDS-PAGE

100 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Mouse EphB3 Fc Chimera at 2 µg/mL (100 µL/well) can bind recombinant mouse Ephrin-B1 Fc Chimera with a linear range of 0.078-1.25 ng/mL.

Formulation, Preparation and Storage

432-B3
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphB3

EphB3, also known as Cek10, Tyro6, Sek4, Hek2, and Mdk5, is a 130 kDa member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1). The 525 amino acid (aa) extracellular domain (ECD) of mature mouse EphB3 contains a ligand binding domain followed by a cysteine rich region and two fibronectin type III domains. The 418 aa cytoplasmic domain contains a tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif (2). Within the ECD, mouse EphB3 shares 96% and 99% aa sequence identity with human and rat EphB3, respectively. Binding of EphB3 to its ligands Ephrin-B1, B2, and B3 triggers forward signaling through EphB3 as well as reverse signaling through the Ephrin (1, 3). EphB3 also interacts in cis with the receptor tyrosine kinase Ryk (4). Activation of its kinase is required for some but not all of the effects of EphB3 on cellular adhesion, motility, and morphology (5). EphB3 is widely expressed during development and in the adult; it shows a complementary tissue distribution to the Ephrin-B ligands (6-9). EphB3 function is important in vascular, nervous system, thymocyte, and palate development (6, 7, 10-12). It directs embyronic neuronal axon pathfinding, and its up-regulation on local macrophages following neuronal injury promotes the growth of regenerating axons (10, 13). EphB3 inhibits colorectal carcinogenesis and invasion by preventing the migration of tumor cells out of the intestinal crypt (9, 14). In non-small cell lung cancer, however, it is up-regulated and can promote tumor progression (15). EphB3 function is supported by the cooperative action of EphB2 in several of these processes (6, 10-12, 16).

References

  1. Park, I. and H.S. Lee (2015) Mol. Cells 38:14.
  2. Ruiz, J.C. et al. (1994) Mech. Dev. 48:153.
  3. Pasquale, E.B (2004) Nat. Neurosci. 7:417.
  4. Trivier, E. and T.S. Ganesan (2002) J. Biol. Chem. 277:23037.
  5. Miao, H. et al. (2005) J. Biol. Chem. 280:923.
  6. Adams, R.H. et al. (1999) Genes Dev. 13:295.
  7. Krull, C.E. et al. (1997) Curr. Biol. 7:571.
  8. Willson, C.A. et al. (2006) J. Mol. Histol. 37:369.
  9. Cortina, C. et al. (2007) Nature Genet. 39:1376.
  10. Birgbauer, E. et al. (2000) Development 127:1231.
  11. Alfaro, D. et al. (2008) Immunology 125:131.
  12. Risley, M. et al. (2009) Mech. Dev. 126:230.
  13. Liu, X. et al. (2006) J. Neurosci. 26:3087.
  14. Batlle, E. et al. (2005) Nature 435:1126.
  15. Ji, X.-D. et al. (2011) Cancer Res. 71:1156.
  16. Holmberg, J. et al. (2006) Cell 125:1151.

Long Name

Eph Receptor B3

Alternate Names

Cek10, Hek2, Mdk5, Sek4, Tyro6

Entrez Gene IDs

2049 (Human); 13845 (Mouse)

Gene Symbol

EPHB3

UniProt

P54754

Additional EphB3 Products

Product Documents for Recombinant Mouse EphB3 Fc Chimera Protein, CF

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