Recombinant Mouse His6-USP18 Protein, CF

Name: Recombinant Mouse His6-USP18 Protein, CF
Brand: R&D Systems
SKU: E-604

R&D Systems, part of Bio-Techne | Catalog # E-604

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Product Datasheet / COA / SDS

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Key Product Details

Source:	T. ni (baculovirus)
Accession #:	AAD21222.1
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Mouse His6-USP18 Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Mouse His6-USP18 Protein, CF

Source

Trichoplusia ni, T. ni (baculovirus)-derived mouse USP18 protein
Ser47 - Ser368 with a N-terminal Met and 6-His tag

Purity

>98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

38 kDa

SDS-PAGE

38 kDa

Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant murine USP18 concentration of 100-250 nM when using ISG15-AMC (Catalog # 212-GD) as a substrate.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-604

Formulation	Supplied as a solution in HEPES, NaCl, Glycerol, EDTA and DTT.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

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Desired Reconstitution Concentration

Background: USP18

Ubiquitin-like carboxyl-terminal hydrolase 18 (USP18) is a specialized cysteine protease with a predicted molecular weight of 42 kDa. USP18 from Mus musculus is a member of the peptidase C19 family and shares 70% amino acid identity with its human ortholog. USP18 plays and important role in antiviral responses and interferon-mediated signaling pathways. Mice lacking the gene (USP18^-/-) are hypersensitive to interferon, have shortened lifespans and pathologic abnormalaties of the brain. While it was initially speculated that the cause of these phenotypes stems from the accumulaiton of ISG15-conjugated intracellular proteins (a target for USP18 catalytic function), it was later demonstrated that USP18 has multiple roles, at least one of which is independent of its ISGylase activity.

Item	Value
References	Goldmann, T. et al. (2015) EMBO J. 34:1612. Ketscher, L. and K.P. Knobeloch (2015) Cytokine 76:569. Knobeloch, K.P. et al. (2005) Mol. Cell Biol. 25:11030. Speer, S.D. et al. (2016) Nat. Commun. 7:11496.
Long Name	Ubiquitin Specific Peptidase 18
Entrez Gene IDs	11274 (Human); 24110 (Mouse); 312688 (Rat)
Alternate Names	EC 3.1.2.15, EC 3.4.19.-, ISG15-specific-processing protease, ISG43, ISG43UBP43, UBP43, USP18, Ubl thiolesterase 18, hUBP43, ubiquitin specific peptidase 18, ubiquitin specific protease 18, ubl carboxyl-terminal hydrolase 18, ubl thioesterase 18,43 kDa ISG15-specific protease

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Recombinant Mouse His6-USP18 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # E-604

Key Product Details

Product Specifications for Recombinant Mouse His6-USP18 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: USP18

Customer Reviews for Recombinant Mouse His6-USP18 Protein, CF

Product Documents for Recombinant Mouse His6-USP18 Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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