Recombinant Mouse Sphingosine Kinase 2/SPHK2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6058-SK

R&D Systems, part of Bio-Techne
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6058-SK-010
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Q9JIA7

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Sphingosine Kinase 2/SPHK2 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Sphingosine Kinase 2/SPHK2 protein
Ala2-Ala617, with an N-terminal Met and an N-terminal 7-His tag
Accession # Q9JIA7

Purity

>70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Inconclusive result, N-terminal Met predicted. Identity confirmed by N-terminal fragments.

Predicted Molecular Mass

67 kDa

SDS-PAGE

68-72 kDa, reducing conditions

Activity

Measured by its ability to phosphorylate Omega-(7-nitro-2-1,3-benzoxadiazol-4-yl)-D-erythro-sphingosine (NBD-sphingosine).
The specific activity is >125 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6058-SK
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT, Glycerol and Tween® 20.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Sphingosine Kinase 2/SPHK2

Sphingosine kinases catalyze the phosphorylation of sphingosine to sphingosine-1-phosphate, a lipid messenger molecule involved in the regulation of processes such as growth, differentiation, and cellular migration (1). Two types of sphingosine kinases, SPHK1 and SPHK2, are known to be expressed in human cells. The two enzymes share considerable amino acid sequence similarity, but differ in their N-terminal and central regions (2). The two proteins also differ in tissue distribution and some kinetic properties (2). Sphingosine kinases can be either cytosolic or membrane-associated. Differences in the distribution of SPHK1 and SPHK2 within cells may be the cause of differences that have been observed in the function of the two enzymes, particularly their roles in apoptosis (3). Sphingosine kinases are considered to be therapeutic targets for cancer and immune system disorders (4, 5).

References

  1. Spiegel, S. (1999) J. Leukocyte Biol. 65:341.
  2. Liu, H. et al. (2000) J. Biol. Chem. 275:19513.
  3. Maceyka, M. et al. (2005) J. Biol. Chem. 280:37118.
  4. Shida, D. et al. (2008) Curr. Drug Targets 9:622.
  5. Melendez, A.J. (2008) Biochim. Biophys. Acta 1784:66.

Alternate Names

SK2, SPK2

Entrez Gene IDs

56848 (Human); 56632 (Mouse); 308589 (Rat)

Gene Symbol

SPHK2

UniProt

Q9JIA7

Additional Sphingosine Kinase 2/SPHK2 Products

Product Documents for Recombinant Mouse Sphingosine Kinase 2/SPHK2 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse Sphingosine Kinase 2/SPHK2 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd. Tween is a registered trademark of ICI Americas. Triton is a registered trademark of Union Carbide Corp.

For research use only

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