Recombinant Human USP3 GST (N-Term) Protein

Name: Recombinant Human USP3 GST (N-Term) Protein
Brand: Novus Biologicals
SKU: H00009960-P02

Novus Biologicals, part of Bio-Techne | Catalog # H00009960-P02

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Product Datasheet / COA / SDS

Discontinued Product

H00009960-P02 has been discontinued. View all USP3 products.

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Wheat germ

Tag

GST (N-Term)

Conjugate

Unconjugated

Applications

ELISA, Affinity Purification, Microarray, Western Blot

View all USP3 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-520 of Human USP3

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MECPHLSSSVCIAPDSAKFPNGSPSSWCCSVCRSNKSPWVCLTCSSVHCGRYVNGHAKKHYEDAQVPLTNHKKSEKQDKVQHTVCMDCSSYSTYCYRCDDFVVNDTKLGLVQKVREHLQNLENSAFTADRHKKRKLLENSTLNSKLLKVNGSTTAICATGLRNLGNTCFMNAILQSLSNIEQFCCYFKELPAVELRNGKTAGRRTYHTRSQGDNNVSLVEEFRKTLCALWQGSQTAFSPESLFYVVWKIMPNFRGYQQQDAHEFMRYLLDHLHLELQGGFNGVSRSAILQENSTLSASNKCCINGASTVVTAIFGGILQNEVNCLICGTESRKFDPFLDLSLDIPSQFRSKRSKNQENGPVCSLRDCLRSFTDLEELDETELYMCHKCKKKQKSTKKFWIQKLPKVLCLHLKRFHWTAYLRNKVDTYVEFPLRGLDMKCYLLEPENSGPESCLYDLAAVVVHHGSGVGSGHYTAYATHEGRWFHFNDSTVTLTDEETVVKAKAYILFYVEHQAKAGSDKL

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

85.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human USP3 GST (N-Term) Protein

12.5% SDS-PAGE Stained with Coomassie Blue.

Formulation, Preparation and Storage

H00009960-P02

Preparation Method	in vitro wheat germ expression system
Formulation	50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: USP3

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 3 (USP3) is a member of this family and is involved in the deubiquitination of histones H2A and H2B. Alternate names for USP3 include ubiquitin carboxyl-terminal hydrolase 3, ubiquitin thioesterase 3, ubiquitin-specific-processing protease 3, and deubiquitinating enzyme 3.

Long Name

Ubiquitin carboxyl-terminal hydrolase 3

Alternate Names

Deubiquitinating enzyme 3, EC 3.1.2.15, EC 3.4.19.12, MGC129878, MGC129879, SIH003, ubiquitin carboxyl-terminal hydrolase 3, ubiquitin specific peptidase 3, ubiquitin specific protease 3, ubiquitin thioesterase 3, Ubiquitin thiolesterase 3, Ubiquitin-specific-processing protease 3, UBP

Gene Symbol

USP3

Additional USP3 Products

Product Documents for Recombinant Human USP3 GST (N-Term) Protein

Product Datasheets

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COA

SDS

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Product Specific Notices for Recombinant Human USP3 GST (N-Term) Protein

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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