Goat anti-Rat IgG (H+L) Secondary Antibody [Alkaline Phosphatase]
Novus Biologicals, part of Bio-Techne | Catalog # NB7113
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
ELISA, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Alkaline Phosphatase
Antibody Source
Polyclonal Goat IgG
Concentration
1 mg/ml
Product Summary for Goat anti-Rat IgG (H+L) Secondary Antibody [Alkaline Phosphatase]
Immunogen
This Goat anti-Rat IgG (H+L) Secondary Antibody [Alkaline Phosphatase] was developed against rat IgG-heavy and light chain.
Specificity
By immunoelectrophoresis and ELISA this Goat anti-Rat IgG (H+L) Secondary Antibody [Alkaline Phosphatase] reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Goat anti-Rat IgG (H+L) Secondary Antibody [Alkaline Phosphatase]
Application
Recommended Usage
ELISA
1:500 to 1:5 000
Immunohistochemistry
1:200 - 1:1
Western Blot
1:100-1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 containing 0.2%BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: IgG (H+L)
The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).
References
1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211
2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78
3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268
4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619
Additional IgG (H+L) Products
Product Documents for Goat anti-Rat IgG (H+L) Secondary Antibody [Alkaline Phosphatase]
Product Specific Notices for Goat anti-Rat IgG (H+L) Secondary Antibody [Alkaline Phosphatase]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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