Goat anti-Rat IgG2a Secondary Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69582
Key Product Details
Species Reactivity
Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen
Label
HRP
Antibody Source
Polyclonal Goat
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Pools of purified homogenous IgG2a isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Reactivity Notes
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this product has not been tested in detail, however in double radial immunodiffusion a weak reaction with Mouse has been observed.
Specificity
The reactivity of the antiserum is directed to the subclass IgG2a as tested against rat sera and a panel of purified homogenous immunoglobulins. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Further tested on the level of sensitivity of direct and indirect immunofluorescence staining methods applied to lymphoid cells. When used on vital peripheral blood cells this immunoconjugate does not react with Fc receptors present on cell surface membranes.
Clonality
Polyclonal
Host
Goat
Applications for Goat anti-Rat IgG2a Secondary Antibody [HRP]
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Application Notes
(In)direct immunofluorescence.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Ion exchange chromatography
Reconstitution
Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2).
Formulation
Lyophilized from PBS (pH 7.2)
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG2A
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G2A Fc
Additional IgG2A Products
Product Documents for Goat anti-Rat IgG2a Secondary Antibody [HRP]
Product Specific Notices for Goat anti-Rat IgG2a Secondary Antibody [HRP]
The lyophilized conjugate is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Diluted antiserum should be stored at 4C, not refrozen, and preferably used the same day.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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