Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Concentration

1.0 mg/ml

View all Lambda Light Chain Secondary Antibodies »

Product Summary for Sheep anti-Human Lambda Light Chain Secondary Antibody

Immunogen

Human Lambda Free Light Chain

Specificity

By immunoelectrophoresis and ELISA this reacts specifically with lambda free light chains. No was detected against kappa light chain or other serum proteins.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Sheep anti-Human Lambda Light Chain Secondary Antibody

Application
Recommended Usage

ELISA

1:1000-1:30000 (Primary) 1:100-1:500 (For Coating Plates)

Immunocytochemistry/ Immunofluorescence

1:200- 1:2000

Immunohistochemistry

1:200- 1:2000

Immunohistochemistry-Paraffin

1:200-1:2000

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Phosphate Buffered Saline (PBS)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Lambda Light Chain

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). This antibody stains only lambda light chain containing cells in human lymphoid tissues.

Alternate Names

IGLC, immunoglobulin lambda constant group

Gene Symbol

IGLC1

Additional Lambda Light Chain Products

Product Documents for Sheep anti-Human Lambda Light Chain Secondary Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Sheep anti-Human Lambda Light Chain Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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