Non-Human Primate BDNF Cartridge

Name: Non-Human Primate BDNF Cartridge
Brand: ProteinSimple
SKU: SPCKB-NP-011279

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-NP-011279

Simple Plex Non-Human Primate BDNF assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), Platelet-poor Plasma (25 uL)
Sensitivity:	5.25 pg/mL
Assay Range:	9.44 - 36,000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor Plasma)
Non-Human Primate BDNF Cartridge Summary:	Simple Plex assay for the detection of non-human primate free Brain-Derived Neurotrophic Factor (BDNF) in cell culture supernatant (CCS), serum and platelet-poor EDTA plasma.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Technical Data
Citations

Product Specifications for Non-Human Primate BDNF Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Non-human Primate
Specificity	Natural and recombinant free BDNF.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, Platelet-poor Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	20	22
Mean (pg/mL)	207	10079	219	11200
Standard Deviation	19.8	867	22.9	1144
CV%	9.6	8.6	10.5	10.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=2)	97	94-101

Linearity

Samples containing and/or spiked with high concentrations of BDNF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Non-Human Primate BDNF Cartridge

	Non-Human Primate BDNF Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BDNF

BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.

Item	Value
Long Name	Brain-derived Neurotrophic Factor
Entrez Gene IDs	627 (Human); 12064 (Mouse); 24225 (Rat)
Alternate Names	ANON2, Abrineurin, BDNF, BULN2, MGC34632, Neurotrophin, brain-derived neurotrophic factor

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Non-Human Primate BDNF Cartridge

Product Datasheets

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Protocols & Manuals

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